Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk

Five strains, designated WS 4672^T, WS 4998, WS 4992^T, WS 4997 and WS 5000, isolated from bovine raw milk formed two individual groups in a phylogenetic analysis. The most similar species on the basis of 16S rRNA gene sequences were Pseudomonas azotoformans IAM 1603^T, Pseudomonas gessardii CIP 105469^Tand Pseudomonas libanensis CIP 105460^Tshowing 99.7–99.6%similarity. Using rpoD gene sequences Pseudomonas veronii LMG 17761^T(93.3 %) was most closely related to strain WS 4672^Tand Pseudomonas libanensis CIP 105460^Tto strain WS 4992^T(93.3 %). The five strains could be differentiated from their closest relatives and from each other by phenotypic and chemotaxonomic characterization and ANIb values calculated from draft genome assemblies. ANIb values of strains WS 4992^Tand WS4671^Tto the closest relatives are lower than 90 %. The major cellular polar lipids of both strains are phosphatidylethanolamine, phosphatidylglycerol, a phospholipid and diphosphatidylglycerol, and their major qui is Q-9. The DNA G+C content of strains WS 4992^Tand WS 4672^Twere 60.0 and 59.7 mol%, respectively. Based on these genotypic and phenotypic traits two novel species of the genus Pseudomonas are proposed: Pseudomonas lactis sp. nov. [with type strain WS 4992^T(=DSM 29167^T=LMG 28435^T) and the additional strains WS 4997 and WS 5000], and Pseudomonas paralactis sp. nov. [with type strain WS 4672^T(=DSM 29164^T=LMG 28439^T) and additional strain WS 4998].