TY - JOUR
T1 - Osteoporosis imaging
T2 - Effects of bone preservation on MDCT-based trabecular bone microstructure parameters and finite element models
AU - Baum, Thomas
AU - Grande Garcia, Eduardo
AU - Burgkart, Rainer
AU - Gordijenko, Olga
AU - Liebl, Hans
AU - Jungmann, Pia M.
AU - Gruber, Michael
AU - Zahel, Tina
AU - Rummeny, Ernst J.
AU - Waldt, Simone
AU - Bauer, Jan S.
N1 - Publisher Copyright:
© 2015 Baum et al.
PY - 2015/6/26
Y1 - 2015/6/26
N2 - Background: Osteoporosis is defined as a skeletal disorder characterized by compromised bone strength due to a reduction of bone mass and deterioration of bone microstructure predisposing an individual to an increased risk of fracture. Trabecular bone microstructure analysis and finite element models (FEM) have shown to improve the prediction of bone strength beyond bone mineral density (BMD) measurements. These computational methods have been developed and validated in specimens preserved in formalin solution or by freezing. However, little is known about the effects of preservation on trabecular bone microstructure and FEM. The purpose of this observational study was to investigate the effects of preservation on trabecular bone microstructure and FEM in human vertebrae. Methods: Four thoracic vertebrae were harvested from each of three fresh human cadavers (n = 12). Multi-detector computed tomography (MDCT) images were obtained at baseline, 3 and 6 month follow-up. In the intervals between MDCT imaging, two vertebrae from each donor were formalin-fixed and frozen, respectively. BMD, trabecular bone microstructure parameters (histomorphometry and fractal dimension), and FEM-based apparent compressive modulus (ACM) were determined in the MDCT images and validated by mechanical testing to failure of the vertebrae after 6 months. Results: Changes of BMD, trabecular bone microstructure parameters, and FEM-based ACM in formalin-fixed and frozen vertebrae over 6 months ranged between 1.0-5.6 % and 1.3-6.1 %, respectively, and were not statistically significant (p > 0.05). BMD, trabecular bone microstructure parameters, and FEM-based ACM as assessed at baseline, 3 and 6 month follow-up correlated significantly with mechanically determined failure load (r = 0.89-0.99; p < 0.05). The correlation coefficients r were not significantly different for the two preservation methods (p > 0.05). Conclusions: Formalin fixation and freezing up to six months showed no significant effects on trabecular bone microstructure and FEM-based ACM in human vertebrae and may both be used in corresponding in-vitro experiments in the context of osteoporosis.
AB - Background: Osteoporosis is defined as a skeletal disorder characterized by compromised bone strength due to a reduction of bone mass and deterioration of bone microstructure predisposing an individual to an increased risk of fracture. Trabecular bone microstructure analysis and finite element models (FEM) have shown to improve the prediction of bone strength beyond bone mineral density (BMD) measurements. These computational methods have been developed and validated in specimens preserved in formalin solution or by freezing. However, little is known about the effects of preservation on trabecular bone microstructure and FEM. The purpose of this observational study was to investigate the effects of preservation on trabecular bone microstructure and FEM in human vertebrae. Methods: Four thoracic vertebrae were harvested from each of three fresh human cadavers (n = 12). Multi-detector computed tomography (MDCT) images were obtained at baseline, 3 and 6 month follow-up. In the intervals between MDCT imaging, two vertebrae from each donor were formalin-fixed and frozen, respectively. BMD, trabecular bone microstructure parameters (histomorphometry and fractal dimension), and FEM-based apparent compressive modulus (ACM) were determined in the MDCT images and validated by mechanical testing to failure of the vertebrae after 6 months. Results: Changes of BMD, trabecular bone microstructure parameters, and FEM-based ACM in formalin-fixed and frozen vertebrae over 6 months ranged between 1.0-5.6 % and 1.3-6.1 %, respectively, and were not statistically significant (p > 0.05). BMD, trabecular bone microstructure parameters, and FEM-based ACM as assessed at baseline, 3 and 6 month follow-up correlated significantly with mechanically determined failure load (r = 0.89-0.99; p < 0.05). The correlation coefficients r were not significantly different for the two preservation methods (p > 0.05). Conclusions: Formalin fixation and freezing up to six months showed no significant effects on trabecular bone microstructure and FEM-based ACM in human vertebrae and may both be used in corresponding in-vitro experiments in the context of osteoporosis.
KW - Bone preservation
KW - Finite element model
KW - Osteoporosis
KW - Trabecular bone microstructure
UR - http://www.scopus.com/inward/record.url?scp=84932116005&partnerID=8YFLogxK
U2 - 10.1186/s12880-015-0066-z
DO - 10.1186/s12880-015-0066-z
M3 - Article
C2 - 26113362
AN - SCOPUS:84932116005
SN - 1471-2342
VL - 15
JO - BMC Medical Imaging
JF - BMC Medical Imaging
IS - 1
M1 - 22
ER -