Abstract
Mouse myotubes were used to investigate effects of the nondepolarizing neuromuscular blocking drugs pancuronium and atracurium on embryonic-type nicotinic acetylcholine receptor channels. Experiments were performed using patch-clamp techniques in combination with devices for ultra-fast solution exchange at outside-out patches. Application of 0.1 mM acetylcholine resulted in a fast current transient. When the peak amplitude was achieved, the current decayed monoexponentially due to desensitization. After application of drugs (pancuronium or atracurium), two different mechanisms of block were observed: (1) open channel block of embryonic-type nicotinic acetylcholine receptor channels after coapplication of blocker and acetylcholine, characterized by decrease of the time constant of current decay; (2) competitive block of embryonic-type nicotinic acetylcholine receptor channels by pancuronium or atracurium after preincubation of outside-out patches with the respective blocker. Different affinities of pancuronium (KB≈0.01 μM) and atracurium (KB≈1 μM) to embryonic-type nicotinic acetylcholine receptor channels were observed.
Originalsprache | Englisch |
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Seiten (von - bis) | 31-35 |
Seitenumfang | 5 |
Fachzeitschrift | European Journal of Pharmacology |
Jahrgang | 413 |
Ausgabenummer | 1 |
DOIs | |
Publikationsstatus | Veröffentlicht - 9 Feb. 2001 |