Neuroendocrine neoplasms of the lung are not associated with point mutations at codon 12 of the Ki-ras gene

Stephan N. Wagner, Rupert Müller, Joachim Boehm, Barbara Pütz, Peter H. Wünsch, Heinz Höfler

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

22 Zitate (Scopus)

Abstract

The most prominent abnormality of ras proto-oncogenes in human lung tumours has involved point mutations at codon 12 of the Ki-ras gene. We have analysed 35 tumour samples of neuroendocrine lung neoplasms (ten carcinoid tumours, ten well-differentiated neuroendocrine carcinomas, and 15 intermediate/small cell neuroendocrine carcinomas) for a point mutation at this site. For this purpose, formalin-fixed and paraffin-embedded tissue sections were microdissected to remove non-tumorous areas. DNA in the remaining tumour tissue was amplified in vitro by the polymerase chain reaction (PCR) and double-stranded PCR products were subjected to sequence analysis. Neither point mutations at codon 12 nor additional structural alterations at codons 1-32 were detected in the Ki-ras gene. Our results suggest that point mutations at codon 12 of the Ki -ras gene do not seem to be involved in the pathogenesis of pulmonary neuroendocrine neoplasms.

OriginalspracheEnglisch
Seiten (von - bis)325-329
Seitenumfang5
FachzeitschriftVirchows Archiv B Cell Pathology Including Molecular Pathology
Jahrgang63
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - Dez. 1993

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