Naturally occurring opioid peptides modulate H+-production by isolated rat parietal cells

W. Schepp, J. Schneider, V. Schusdziarra, M. Classen

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

5 Zitate (Scopus)

Abstract

The rationale for the present study was to determine the effects of naturally occurring opioid peptides on H+-production by isolated rat parietal cells as indirectly measured by [14C]-aminopyrine uptake. In crude preparations (18 to 25% parietal cells) and in enriched (80 to 90%) parietal cell fractions stimulation by submaximal histamine- or dibutyryl cAMP-concentrations (10-6-10-4 mol/l) was augmented by 20-30% in the presence of methionine-enkephalin (Met-Enk) and Met-Enk Arg6Phe7 (10-7 to 10-5 mol/l). This augmentation was blocked by the opiate receptor antagonist (-)naloxone (10-6 mol/l) suggesting specificity of the action of Met-Enk and Met-Enk Arg6Phe7. At 10-6 mol/l (-)naloxone did not exert nonspecific toxic effects. Yet, even in the absence of exogenous opioids, histamine-induced H4-production was inhibited by 3×10-5 or 10-4 mol/l (-)naloxone. Since similar inhibition occurred with (+)naloxone, an inactive stereoisomer which does not interact with opiate receptors, effects of (-)naloxone at concentrations above 10-5 mol/l must be considered nonspecific. We conclude that Met-Enk and Met-Enk Arg6Phe7 have no effect on basal, but augment stimulated H+-production by a direct effect on the parietal cells. At nontoxic concentrations (-)naloxone antagonizes this augmentation indicating that it is mediated by specific opiate receptors on the parietal cells.

OriginalspracheEnglisch
Seiten (von - bis)885-890
Seitenumfang6
FachzeitschriftPeptides
Jahrgang7
Ausgabenummer5
DOIs
PublikationsstatusVeröffentlicht - 1986

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