Multiparametric optimization of multispectral optoacoustic tomography for deep tissue imaging

Jürgen Glatz, Nikolaos C. Deliolanis, Lu Ding, Adrian Taruttis, Amir Rosenthal, Ralf Schulz, Daniel Razansky, Vasilis Ntziachristos

Publikation: Beitrag in Buch/Bericht/KonferenzbandKonferenzbeitragBegutachtung

1 Zitat (Scopus)

Abstract

Over the last decade fluorescent reporter technologies (both fluorescent probes and proteins) have become a very powerful imaging tool in everyday biomedical research. Multispectral optoacoustic tomography (MSOT) is an emerging imaging technology that can resolve fluorophore concentration in small animals situated in deep tissue by multispectral acquisition and processing of optoacoustic signals. In this work, we study the optimum operating conditions of MSOT in imaging fluorescence activity in small animals. The performance of various fluorochromes / fluorescent proteins is examined and it is shown that the new infrared fluorescent protein is an order of magnitude brighter than the red ones. Finally, wavelength reduction after principle component analysis shows, that accurate unmixing and 3D reconstruction of the distribution of fluorochromes is possible only with 2 or 3 wavelengths.

OriginalspracheEnglisch
TitelPhotons Plus Ultrasound
UntertitelImaging and Sensing 2010
DOIs
PublikationsstatusVeröffentlicht - 2010
VeranstaltungPhotons Plus Ultrasound: Imaging and Sensing 2010 - San Francisco, CA, USA/Vereinigte Staaten
Dauer: 24 Jan. 201026 Jan. 2010

Publikationsreihe

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Band7564
ISSN (Print)1605-7422

Konferenz

KonferenzPhotons Plus Ultrasound: Imaging and Sensing 2010
Land/GebietUSA/Vereinigte Staaten
OrtSan Francisco, CA
Zeitraum24/01/1026/01/10

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