Multi-spectral photo-acoustic molecular tomography resolves fluorochrome distribution with high resolution and sensitivity in small animals

Publikation: Beitrag in Buch/Bericht/KonferenzbandKonferenzbeitragBegutachtung

2 Zitate (Scopus)

Abstract

Current non-invasive imaging methods of fluorescent molecular probes in the visible and near-infrared suffer from low spatial resolution as a result of rapid light diffusion in biological tissues. We show that three-dimensional distribution of fluorochromes deep in small animals can be resolved with below 25 femtomole sensitivity and 150 microns spatial resolution by means of multi-spectral photoacoustic molecular tomography. The low sensitivity limit of the method is enabled by using the highly resonant absorption spectrum of a commonly used near-infrared fluorescent molecular probe Alexa Fluor® 750 in order to acquire differential images at multiple wavelengths with tomographic topology suitable for whole-body small animal imaging.

OriginalspracheEnglisch
TitelPhotons Plus Ultrasound
UntertitelImaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
DOIs
PublikationsstatusVeröffentlicht - 2008
Veranstaltung9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008 - San Jose, CA, USA/Vereinigte Staaten
Dauer: 20 Jan. 200823 Jan. 2008

Publikationsreihe

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Band6856
ISSN (Print)1605-7422

Konferenz

Konferenz9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008
Land/GebietUSA/Vereinigte Staaten
OrtSan Jose, CA
Zeitraum20/01/0823/01/08

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