Mouse redox histology using genetically encoded probes

Yuuta Fujikawa, Leticia P. Roma, Mirko C. Sobotta, Adam J. Rose, Mauricio Berriel Diaz, Giuseppe Locatelli, Michael O. Breckwoldt, Thomas Misgeld, Martin Kerschensteiner, Stephan Herzig, Karin Müller-Decker, Tobias P. Dick

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

60 Zitate (Scopus)

Abstract

Mapping the in vivo distribution of endogenous oxidants in animal tissues is of substantial biomedical interest. Numerous health-related factors, including diet, physical activity, infection, aging, toxins, or pharmacological intervention, may cause redox changes. Tools are needed to pinpoint redox state changes to particular organs, tissues, cell types, and subcellular organelles. We describe a procedure that preserves the in vivo redox state of genetically encoded redox biosensors within histological tissue sections, thus providing "redox maps" for any tissue and comparison of interest. We demonstrate the utility of the technique by visualizing endogenous redox differences and changes in the context of tumor growth, inflammation, embryonic development, and nutrient starvation.

OriginalspracheEnglisch
AufsatznummerRS1
FachzeitschriftScience Signaling
Jahrgang9
Ausgabenummer419
DOIs
PublikationsstatusVeröffentlicht - 15 März 2016

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