TY - JOUR
T1 - miREV
T2 - An Online Database and Tool to Uncover Potential Reference RNAs and Biomarkers in Small-RNA Sequencing Data Sets from Extracellular Vesicles Enriched Samples
AU - Hildebrandt, Alex
AU - Kirchner, Benedikt
AU - Nolte-'t Hoen, Esther N.M.
AU - Pfaffl, Michael W.
N1 - Publisher Copyright:
© 2021
PY - 2021/7/23
Y1 - 2021/7/23
N2 - Extracellular vesicles (EVs) are nano-sized, membrane-enclosed vesicles released by cells for intercellular communication. EVs are involved in pathological processes and miRNAs in EVs have gained interest as easily accessible biomolecules in liquid biopsies for diagnostic purposes. To validate potential miRNA biomarker, transcriptome analyses must be carried out to detect suitable reference miRNAs. miREV is a database with over 400 miRNA sequencing data sets and helps the researcher to find suitable reference miRNAs for their individual experimental setup. The researcher can put together a specific sample set in miREV, which is similar to his own experimental concept in order to find the most suitable references. This allows to run validation experiments without having to carry out a complex and costly transcriptome analysis priorly. Additional read count tables of each generated sample set are downloadable for further analysis. miREV is freely available at https://www.physio.wzw.tum.de/mirev/.
AB - Extracellular vesicles (EVs) are nano-sized, membrane-enclosed vesicles released by cells for intercellular communication. EVs are involved in pathological processes and miRNAs in EVs have gained interest as easily accessible biomolecules in liquid biopsies for diagnostic purposes. To validate potential miRNA biomarker, transcriptome analyses must be carried out to detect suitable reference miRNAs. miREV is a database with over 400 miRNA sequencing data sets and helps the researcher to find suitable reference miRNAs for their individual experimental setup. The researcher can put together a specific sample set in miREV, which is similar to his own experimental concept in order to find the most suitable references. This allows to run validation experiments without having to carry out a complex and costly transcriptome analysis priorly. Additional read count tables of each generated sample set are downloadable for further analysis. miREV is freely available at https://www.physio.wzw.tum.de/mirev/.
KW - Extracellular vesicles
KW - Next-generation Sequencing
KW - RT-qPCR
KW - biofluid
KW - human blood plasma & serum
KW - normalization methods
KW - stability algorithms
UR - http://www.scopus.com/inward/record.url?scp=85108609673&partnerID=8YFLogxK
U2 - 10.1016/j.jmb.2021.167070
DO - 10.1016/j.jmb.2021.167070
M3 - Article
C2 - 34052284
AN - SCOPUS:85108609673
SN - 0022-2836
VL - 433
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 15
M1 - 167070
ER -