TY - JOUR
T1 - Mimicking Native Extracellular Matrix with Phytic Acid-Crosslinked Protein Nanofibers for Cardiac Tissue Engineering
AU - Ravichandran, Rajeswari
AU - Seitz, Vera
AU - Reddy Venugopal, Jayarama
AU - Sridhar, Radhakrishnan
AU - Sundarrajan, Subramanian
AU - Mukherjee, Shayanti
AU - Wintermantel, Erich
AU - Ramakrishna, Seeram
PY - 2013/3
Y1 - 2013/3
N2 - A functional scaffold fabricated is developed from natural polymers, favoring regeneration of the ischemic myocardium. Hemoglobin/gelatin/fibrinogen (Hb/gel/fib) nanofibers are fabricated by electrospinning and are characterized for morphology, scaffold composition, functional groups and hydrophilicity. It is hypothesized that ex vivo pretreatment of mesenchymal stem cells (MSCs) using 5-azacytidine and such a functional nanofibrous construct having a high oxygen-carrying potential could lead to enhanced cardiomyogenic differentiation of MSCs and result in superior biological and functional effects. The combination of a functional nanofibrous scaffold composed of natural polymers and crosslinked with a natural crosslinking agent, phytic acid, and stem cell biology may prove to be a novel therapeutic device for treatment of myocardial infarction.
AB - A functional scaffold fabricated is developed from natural polymers, favoring regeneration of the ischemic myocardium. Hemoglobin/gelatin/fibrinogen (Hb/gel/fib) nanofibers are fabricated by electrospinning and are characterized for morphology, scaffold composition, functional groups and hydrophilicity. It is hypothesized that ex vivo pretreatment of mesenchymal stem cells (MSCs) using 5-azacytidine and such a functional nanofibrous construct having a high oxygen-carrying potential could lead to enhanced cardiomyogenic differentiation of MSCs and result in superior biological and functional effects. The combination of a functional nanofibrous scaffold composed of natural polymers and crosslinked with a natural crosslinking agent, phytic acid, and stem cell biology may prove to be a novel therapeutic device for treatment of myocardial infarction.
KW - Cardiogenic differentiation
KW - Crosslinkers
KW - Ischemic myocardium
KW - Mesenchymal stem cells
KW - Phytic acid
UR - http://www.scopus.com/inward/record.url?scp=84875511541&partnerID=8YFLogxK
U2 - 10.1002/mabi.201200391
DO - 10.1002/mabi.201200391
M3 - Article
C2 - 23335565
AN - SCOPUS:84875511541
SN - 1616-5187
VL - 13
SP - 366
EP - 375
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
IS - 3
ER -