Microbiological aspects of determination of trichloroacetic acid in soil

M. Matucha; M. Gryndler; H. Uhlířová; K. Fuksová; J. Rohlenová; S. T. Forczek; P. Schröder

doi:10.1007/BF02931384

Microbiological aspects of determination of trichloroacetic acid in soil

M. Matucha
, M. Gryndler
, H. Uhlířová
, K. Fuksová
, J. Rohlenová
, S. T. Forczek
, P. Schröder

Institute of Experimental Botany of the Academy of Sciences of the Czech Republic
Institute of Microbiology of the Czech Academy of Sciences
Forestry and Game Management Research Institute
Charles University
Helmholtz Zentrum München German Research Center for Environmental Health

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

5 Zitate (Scopus)

Abstract

Soils have been shown to possess a strong microbial trichloroacetic acid (TCA)-degrading activity. High TCA-degradation rate was also observed during soil extraction with water. For correct measurements of TCA levels in soil all TCA-degrading activities have to be inhibited immediately after sampling before analysis. We used rapid freezing of soil samples (optimally in liquid nitrogen) with subsequent storage and slow thawing before analysis as an efficient technique for suppressing the degradation. Frozen soil samples stored overnight at -20°C and then thawed slowly exhibited very low residual TCA-degrading activity for several hours. Omitting the above procedure could lead to the confusing differences between the TCA levels previously reported in the literature.

Originalsprache	Englisch
Seiten (von - bis)	117-122
Seitenumfang	6
Fachzeitschrift	Folia Microbiologica
Jahrgang	49
Ausgabenummer	2
DOIs	https://doi.org/10.1007/BF02931384
Publikationsstatus	Veröffentlicht - 2004
Extern publiziert	Ja

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title = "Microbiological aspects of determination of trichloroacetic acid in soil",

abstract = "Soils have been shown to possess a strong microbial trichloroacetic acid (TCA)-degrading activity. High TCA-degradation rate was also observed during soil extraction with water. For correct measurements of TCA levels in soil all TCA-degrading activities have to be inhibited immediately after sampling before analysis. We used rapid freezing of soil samples (optimally in liquid nitrogen) with subsequent storage and slow thawing before analysis as an efficient technique for suppressing the degradation. Frozen soil samples stored overnight at -20°C and then thawed slowly exhibited very low residual TCA-degrading activity for several hours. Omitting the above procedure could lead to the confusing differences between the TCA levels previously reported in the literature.",

author = "M. Matucha and M. Gryndler and H. Uhl{\'i}{\v r}ov{\'a} and K. Fuksov{\'a} and J. Rohlenov{\'a} and Forczek, \{S. T.\} and P. Schr{\"o}der",

note = "Funding Information: This work was supported by grant 522/02/0874 of the Grant Agency of the Czech Republic. The support of the GSF – National Research Center for Environment and Health (Neuherberg, Germany) is gratefully acknowledged. We thank Mr. J. Fallet (Loeten, Norway) for cooperation in finding a suitable locality for soil sampling and providing appropriate forest soil samples, Dr. L. Lis{\'a} (Institute of Microbiology, Academy of Sciences of the Czech Republic) for the help with cryopreservation and Dr. A. Riedel (Institute of Clinical and Experimental Medicine, Prague) and Dr. S. Pavelka (Institute of Physiology, Academy of Sciences of the Czech Republic) for radioactivity measurements.",

year = "2004",

doi = "10.1007/BF02931384",

language = "English",

volume = "49",

pages = "117--122",

journal = "Folia Microbiologica",

issn = "0015-5632",

publisher = "Springer Science and Business Media B.V.",

number = "2",

}

TY - JOUR

T1 - Microbiological aspects of determination of trichloroacetic acid in soil

AU - Matucha, M.

AU - Gryndler, M.

AU - Uhlířová, H.

AU - Fuksová, K.

AU - Rohlenová, J.

AU - Forczek, S. T.

AU - Schröder, P.

N1 - Funding Information: This work was supported by grant 522/02/0874 of the Grant Agency of the Czech Republic. The support of the GSF – National Research Center for Environment and Health (Neuherberg, Germany) is gratefully acknowledged. We thank Mr. J. Fallet (Loeten, Norway) for cooperation in finding a suitable locality for soil sampling and providing appropriate forest soil samples, Dr. L. Lisá (Institute of Microbiology, Academy of Sciences of the Czech Republic) for the help with cryopreservation and Dr. A. Riedel (Institute of Clinical and Experimental Medicine, Prague) and Dr. S. Pavelka (Institute of Physiology, Academy of Sciences of the Czech Republic) for radioactivity measurements.

PY - 2004

Y1 - 2004

N2 - Soils have been shown to possess a strong microbial trichloroacetic acid (TCA)-degrading activity. High TCA-degradation rate was also observed during soil extraction with water. For correct measurements of TCA levels in soil all TCA-degrading activities have to be inhibited immediately after sampling before analysis. We used rapid freezing of soil samples (optimally in liquid nitrogen) with subsequent storage and slow thawing before analysis as an efficient technique for suppressing the degradation. Frozen soil samples stored overnight at -20°C and then thawed slowly exhibited very low residual TCA-degrading activity for several hours. Omitting the above procedure could lead to the confusing differences between the TCA levels previously reported in the literature.

AB - Soils have been shown to possess a strong microbial trichloroacetic acid (TCA)-degrading activity. High TCA-degradation rate was also observed during soil extraction with water. For correct measurements of TCA levels in soil all TCA-degrading activities have to be inhibited immediately after sampling before analysis. We used rapid freezing of soil samples (optimally in liquid nitrogen) with subsequent storage and slow thawing before analysis as an efficient technique for suppressing the degradation. Frozen soil samples stored overnight at -20°C and then thawed slowly exhibited very low residual TCA-degrading activity for several hours. Omitting the above procedure could lead to the confusing differences between the TCA levels previously reported in the literature.

UR - https://www.scopus.com/pages/publications/2542595607

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DO - 10.1007/BF02931384

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VL - 49

SP - 117

EP - 122

JO - Folia Microbiologica

JF - Folia Microbiologica

IS - 2

ER -

Microbiological aspects of determination of trichloroacetic acid in soil

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