@article{51c39bf0c2c644768a6f9595f604a7a6,
title = "Microbiological aspects of determination of trichloroacetic acid in soil",
abstract = "Soils have been shown to possess a strong microbial trichloroacetic acid (TCA)-degrading activity. High TCA-degradation rate was also observed during soil extraction with water. For correct measurements of TCA levels in soil all TCA-degrading activities have to be inhibited immediately after sampling before analysis. We used rapid freezing of soil samples (optimally in liquid nitrogen) with subsequent storage and slow thawing before analysis as an efficient technique for suppressing the degradation. Frozen soil samples stored overnight at -20°C and then thawed slowly exhibited very low residual TCA-degrading activity for several hours. Omitting the above procedure could lead to the confusing differences between the TCA levels previously reported in the literature.",
author = "M. Matucha and M. Gryndler and H. Uhl{\'i}{\v r}ov{\'a} and K. Fuksov{\'a} and J. Rohlenov{\'a} and Forczek, {S. T.} and P. Schr{\"o}der",
note = "Funding Information: This work was supported by grant 522/02/0874 of the Grant Agency of the Czech Republic. The support of the GSF – National Research Center for Environment and Health (Neuherberg, Germany) is gratefully acknowledged. We thank Mr. J. Fallet (Loeten, Norway) for cooperation in finding a suitable locality for soil sampling and providing appropriate forest soil samples, Dr. L. Lis{\'a} (Institute of Microbiology, Academy of Sciences of the Czech Republic) for the help with cryopreservation and Dr. A. Riedel (Institute of Clinical and Experimental Medicine, Prague) and Dr. S. Pavelka (Institute of Physiology, Academy of Sciences of the Czech Republic) for radioactivity measurements.",
year = "2004",
doi = "10.1007/BF02931384",
language = "English",
volume = "49",
pages = "117--122",
journal = "Folia Microbiologica",
issn = "0015-5632",
publisher = "Springer Science and Business Media B.V.",
number = "2",
}