TY - JOUR
T1 - Method for toxicity test of titanium dioxide nanoparticles in ciliate protozoan Tetrahymena
AU - Ud-Daula, Asad
AU - Pfister, Gerd
AU - Schramm, Karl Werner
N1 - Funding Information:
This research was supported and funded by Helmholtz Zentrum München-German Research Center for Environmental Health, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany. The authors want to thank to Tetrahymena Stock Center, C5 155 Veterinary Medical Center, Cornell University, Ithaca, New York, for providing Tetrahymena thermophila cell line.
PY - 2013/9/19
Y1 - 2013/9/19
N2 - Titanium dioxide (TiO2) nanoparticles have a high surface-to-mass ratio and rapidly aggregate in water causing great difficulties for toxicity test exposed to aquatic organisms or other cell lines. This study uses a cell viability kit for routine toxicity test of TiO2 as well as other nanoparticles which accumulate in the aquatic environment. Tetrahymena immediately endocytoses TiO2 nanoparticles and stores them in food vacuoles until the particles undergo exocytosis as larger aggregates. However, during the process of endocytosis and exocytosis, TiO2 particles interfere with cell growth and consequently induce acute toxicity. It exerted high cell growth inhibition at 20 h incubation and induces significant cytotoxic effects. Surprisingly, the effect of TiO2 decreases at 40 h incubation, due to the recovery of cell growth and reduction of the cytotoxicity of the particles.
AB - Titanium dioxide (TiO2) nanoparticles have a high surface-to-mass ratio and rapidly aggregate in water causing great difficulties for toxicity test exposed to aquatic organisms or other cell lines. This study uses a cell viability kit for routine toxicity test of TiO2 as well as other nanoparticles which accumulate in the aquatic environment. Tetrahymena immediately endocytoses TiO2 nanoparticles and stores them in food vacuoles until the particles undergo exocytosis as larger aggregates. However, during the process of endocytosis and exocytosis, TiO2 particles interfere with cell growth and consequently induce acute toxicity. It exerted high cell growth inhibition at 20 h incubation and induces significant cytotoxic effects. Surprisingly, the effect of TiO2 decreases at 40 h incubation, due to the recovery of cell growth and reduction of the cytotoxicity of the particles.
KW - Cell growth inhibition
KW - Cell viability kits
KW - Cytotoxicity
KW - Effective concentration
KW - Tetrahymena thermophila
KW - TiO nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84878825449&partnerID=8YFLogxK
U2 - 10.1080/10934529.2013.781878
DO - 10.1080/10934529.2013.781878
M3 - Article
C2 - 23705610
AN - SCOPUS:84878825449
SN - 1093-4529
VL - 48
SP - 1343
EP - 1348
JO - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
JF - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
IS - 11
ER -