TY - JOUR
T1 - Metabolite alterations and interactions with microbiota in helicobacter pylori-Associated gastric lesions
AU - Peng, Lei
AU - Guo, Yang
AU - Gerhard, Markus
AU - Gao, Juan Juan
AU - Liu, Zong Chao
AU - Mejías-Luque, Raquel
AU - Zhang, Lian
AU - Vieth, Michael
AU - Ma, Jun Ling
AU - Liu, Wei Dong
AU - Li, Zhe Xuan
AU - Zhou, Tong
AU - Li, Wen Qing
AU - You, Wei Cheng
AU - Zhang, Yang
AU - Pan, Kai Feng
N1 - Publisher Copyright:
© 2023 American Society for Microbiology. All rights reserved.
PY - 2023/8
Y1 - 2023/8
N2 - Metabolites and their interactions with microbiota may be involved in Helicobacter pylori-Associated gastric lesion development. This study aimed to explore metabolite alterations upon H. pylori eradication and possible roles of microbiota-metabolite interactions in progression of precancerous lesions. Targeted metabolomics assays and 16S rRNA gene sequencing were conducted to investigate metabolic and microbial alterations of paired gastric biopsy specimens in 58 subjects with successful and 57 subjects with failed anti-H. pylori treatment. Integrative analyses were performed by combining the metabolomics and microbiome profiles from the same intervention participants. A total of 81 metabolites were significantly altered after successful eradication compared to failed treatment, including acylcarnitines, ceramides, triacylglycerol, cholesterol esters, fatty acid, sphingolipids, glycerophospholipids, and glycosylceramides, with P values of ,0.05 for all. The differential metabolites showed significant correlations with microbiota in baseline biopsy specimens, such as negative correlations between Helicobacter and glycerophospholipids, glycosylceramide, and triacylglycerol (P , 0.05 for all), which were altered by eradication. The characteristic negative correlations between glycosylceramides and Fusobacterium, Streptococcus, and Gemella in H. pylori-positive baseline biopsy specimens were further noticed in active gastritis and intestinal metaplasia (P , 0.05 for all). A panel including differential metabolites, genera, and their interactions may help to discriminate high-risk subjects who progressed from mild to advanced precancerous lesions in short-Term and long-Term follow-up periods with areas under the curve (AUC) of 0.914 and 0.801, respectively. Therefore, our findings provide new insights into the metabolites and microbiota interactions in H. pylori-Associated gastric lesion progression. IMPORTANCE In this study, a panel was established including differential metabolites, genera, and their interactions, which may help to discriminate high-risk subjects for progression from mild lesions to advanced precancerous lesions in short-Term and long-Term follow-up.
AB - Metabolites and their interactions with microbiota may be involved in Helicobacter pylori-Associated gastric lesion development. This study aimed to explore metabolite alterations upon H. pylori eradication and possible roles of microbiota-metabolite interactions in progression of precancerous lesions. Targeted metabolomics assays and 16S rRNA gene sequencing were conducted to investigate metabolic and microbial alterations of paired gastric biopsy specimens in 58 subjects with successful and 57 subjects with failed anti-H. pylori treatment. Integrative analyses were performed by combining the metabolomics and microbiome profiles from the same intervention participants. A total of 81 metabolites were significantly altered after successful eradication compared to failed treatment, including acylcarnitines, ceramides, triacylglycerol, cholesterol esters, fatty acid, sphingolipids, glycerophospholipids, and glycosylceramides, with P values of ,0.05 for all. The differential metabolites showed significant correlations with microbiota in baseline biopsy specimens, such as negative correlations between Helicobacter and glycerophospholipids, glycosylceramide, and triacylglycerol (P , 0.05 for all), which were altered by eradication. The characteristic negative correlations between glycosylceramides and Fusobacterium, Streptococcus, and Gemella in H. pylori-positive baseline biopsy specimens were further noticed in active gastritis and intestinal metaplasia (P , 0.05 for all). A panel including differential metabolites, genera, and their interactions may help to discriminate high-risk subjects who progressed from mild to advanced precancerous lesions in short-Term and long-Term follow-up periods with areas under the curve (AUC) of 0.914 and 0.801, respectively. Therefore, our findings provide new insights into the metabolites and microbiota interactions in H. pylori-Associated gastric lesion progression. IMPORTANCE In this study, a panel was established including differential metabolites, genera, and their interactions, which may help to discriminate high-risk subjects for progression from mild lesions to advanced precancerous lesions in short-Term and long-Term follow-up.
KW - Gastric metabolites
KW - Gastric microbiota
KW - Helicobacter pylori
KW - Interactions
KW - Precancerous lesions
UR - http://www.scopus.com/inward/record.url?scp=85168235802&partnerID=8YFLogxK
U2 - 10.1128/spectrum.05347-22
DO - 10.1128/spectrum.05347-22
M3 - Article
C2 - 37358459
AN - SCOPUS:85168235802
SN - 2165-0497
VL - 11
JO - Microbiology Spectrum
JF - Microbiology Spectrum
IS - 4
ER -