Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus

A magnetic capture-hybridization method was assessed for the isolation of prokaryotic mRNA for the neutral protease of B. cereus from liquid culture. A biotin-labeled specific probe was hybridized to the mRNA transcripts and subsequently captured by streptavidin-coated paramagnetic beads. mRNA was detected by dot-blot hybridization with a ds DIG-labeled DNA-probe. The magnetic capture hybridization is a rapid and simple method and has a promising potential for gene expression studies in complex samples. Copyright (C) 1999 Elsevier Science B.V.