Intraluteal release of prostaglandin F and E2 during corpora lutea development in the cow

Shu Ichi Kobayashi, Tomas J. Acosta, Kanako Hayashi, Bajram Berisha, Takahiro Ozawa, Masayuki Ohtani, Dieter Schams, Akio Miyamoto

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

16 Zitate (Scopus)

Abstract

It is well known that prostaglandin (PG) F and PGE 2 are actively produced by bovine early corpus luteum (CL) at higher levels than in the CL of later stages. However, there is no in vivo information about the local secretion of PGs within bovine developing CL so far. Thus, the objective of the present study was to determine in detail the real-time changes in PGF and PGE2 secretion within developing CL. A microdialysis system (MDS) was surgically implanted into the newly formed CL of 6 cows on Day 3 after GnRH administration (about 1.5 days after ovulation) to induce superovulation following FSH treatment. Both PGF and PGE2 release were high during the first 24 h within developing CL but decreased thereafter. These profiles were well reflected in the PG concentrations in ovarian venous plasma. Furthermore, reverse transcription polymerase chain reaction analysis using CL from other animals independent from the MDS study revealed that the level of cyclo-oxyganase-2 mRNA expression in early CL was higher than that of later luteal phases. The present results provide the first direct in vivo evidence that active PGs production in CL occurs up to Day 4 after estrus in the cow.

OriginalspracheEnglisch
Seiten (von - bis)583-590
Seitenumfang8
FachzeitschriftJournal of Reproduction and Development
Jahrgang48
Ausgabenummer6
DOIs
PublikationsstatusVeröffentlicht - 2002

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