Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis

Ejvind Mortz; Thomas N. Krogh; Henrik Vorum; Angelika Görg

doi:10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q

Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis

Ejvind Mortz, Thomas N. Krogh, Henrik Vorum, Angelika Görg

TUM Emeriti of Excellence

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

468 Zitate (Scopus)

Abstract

In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.

Originalsprache	Englisch
Seiten (von - bis)	1359-1363
Seitenumfang	5
Fachzeitschrift	Proteomics
Jahrgang	1
Ausgabenummer	11
DOIs	https://doi.org/10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q
Publikationsstatus	Veröffentlicht - Nov. 2001

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10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q

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abstract = "In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.",

keywords = "Mass spectrometry, Peptide map, Protein identification, Silver stain",

author = "Ejvind Mortz and Krogh, {Thomas N.} and Henrik Vorum and Angelika G{\"o}rg",

year = "2001",

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AU - Vorum, Henrik

AU - Görg, Angelika

PY - 2001/11

Y1 - 2001/11

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KW - Mass spectrometry

KW - Peptide map

KW - Protein identification

KW - Silver stain

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Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis

Abstract

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