Improved Culture System for Microspores of Barley to Become a Target for DNA Uptake

Ursula Kuhlmann, Bärbel Foroughi‐Wehr, A. Graner, G. Wenzel

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

19 Zitate (Scopus)

Abstract

For the winter barley cultivar ‘Igri’, a microspore isolation and regeneration procedure is described which allows the production of such vigorous micro‐spore fractions that this single‐cell system can be used as a target for DNA uptake. Up to 60 % of the vigorous microspores isolated from the anther, and cultured in liquid modified MS medium, formed embryoids and/or calli. Such preparations were used for trials in DNA uptake with the plasmid pBI 221. Transformation trials were performed with polyethylene glycol as the inducing agent. With this treatment, a relative increase of fluorescence could be shown under UV light indicative of transient expression of the uidA gene.

OriginalspracheEnglisch
Seiten (von - bis)165-168
Seitenumfang4
FachzeitschriftPlant Breeding
Jahrgang107
Ausgabenummer2
DOIs
PublikationsstatusVeröffentlicht - Sept. 1991
Extern publiziertJa

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