Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor

Nicola Heim; Olga Garaschuk; Michael W. Friedrich; Marco Mank; Ruxandra I. Milos; Yury Kovalchuk; Arthur Konnerth; Oliver Griesbeck

doi:10.1038/nmeth1009

Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor

Nicola Heim, Olga Garaschuk, Michael W. Friedrich, Marco Mank, Ruxandra I. Milos, Yury Kovalchuk, Arthur Konnerth, Oliver Griesbeck

Default

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

168 Zitate (Scopus)

Abstract

Fluorescent Ca²⁺ indicator proteins (FCIPs) are attractive tools for studying Ca²⁺ dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca²⁺ sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca²⁺ imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.

Originalsprache	Englisch
Seiten (von - bis)	127-129
Seitenumfang	3
Fachzeitschrift	Nature Methods
Jahrgang	4
Ausgabenummer	2
DOIs	https://doi.org/10.1038/nmeth1009
Publikationsstatus	Veröffentlicht - Feb. 2007

Zugriff auf Dokument

10.1038/nmeth1009

Andere Dateien und Links

Verknüpfung zur Publikation in Scopus

Dieses zitieren

@article{906abd37a6014bc49c31cf4bb6046d6a,

title = "Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor",

abstract = "Fluorescent Ca2+ indicator proteins (FCIPs) are attractive tools for studying Ca2+ dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca2+ sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca2+ imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.",

author = "Nicola Heim and Olga Garaschuk and Friedrich, {Michael W.} and Marco Mank and Milos, {Ruxandra I.} and Yury Kovalchuk and Arthur Konnerth and Oliver Griesbeck",

note = "Funding Information: We thank M. B{\"o}sl for doing pronucleus injections, and A. Schulze-Schenke and S. Direnberger for assistance. Supported by grants of the Deutsche Forschungsgemeinschaft (SFB 391 and SFB 596) and the Bundesministerium f{\"u}r Bildung und Forschung (NGFN-2).",

year = "2007",

month = feb,

doi = "10.1038/nmeth1009",

language = "English",

volume = "4",

pages = "127--129",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Research",

number = "2",

}

TY - JOUR

T1 - Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor

AU - Heim, Nicola

AU - Garaschuk, Olga

AU - Friedrich, Michael W.

AU - Mank, Marco

AU - Milos, Ruxandra I.

AU - Kovalchuk, Yury

AU - Konnerth, Arthur

AU - Griesbeck, Oliver

N1 - Funding Information: We thank M. Bösl for doing pronucleus injections, and A. Schulze-Schenke and S. Direnberger for assistance. Supported by grants of the Deutsche Forschungsgemeinschaft (SFB 391 and SFB 596) and the Bundesministerium für Bildung und Forschung (NGFN-2).

PY - 2007/2

Y1 - 2007/2

N2 - Fluorescent Ca2+ indicator proteins (FCIPs) are attractive tools for studying Ca2+ dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca2+ sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca2+ imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.

AB - Fluorescent Ca2+ indicator proteins (FCIPs) are attractive tools for studying Ca2+ dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca2+ sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca2+ imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.

UR - http://www.scopus.com/inward/record.url?scp=33847628425&partnerID=8YFLogxK

U2 - 10.1038/nmeth1009

DO - 10.1038/nmeth1009

M3 - Article

C2 - 17259991

AN - SCOPUS:33847628425

SN - 1548-7091

VL - 4

SP - 127

EP - 129

JO - Nature Methods

JF - Nature Methods

IS - 2

ER -

Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor

Abstract

Zugriff auf Dokument

Andere Dateien und Links

Fingerprint

Dieses zitieren