Imaging-Based Screening Platform Assists Protein Engineering

Arne Fabritius; David Ng; Andreas Michael Kist; Mutlu Erdogan; Ruben Portugues; Oliver Griesbeck

doi:10.1016/j.chembiol.2018.08.008

Imaging-Based Screening Platform Assists Protein Engineering

Arne Fabritius, David Ng, Andreas Michael Kist, Mutlu Erdogan, Ruben Portugues, Oliver Griesbeck

Max Planck Institute of Neurobiology

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

18 Zitate (Scopus)

Abstract

Protein evolution requires generation and screening of large numbers of variants. To expedite screening, Fabritius et al. built a platform that integrates fluorescence-based image analysis and picking of bacterial colonies. They engineered mCarmine, a far-red-emitting fluorescent protein for tagging and deep tissue imaging.

Originalsprache	Englisch
Seiten (von - bis)	1554-1561.e8
Fachzeitschrift	Cell Chemical Biology
Jahrgang	25
Ausgabenummer	12
DOIs	https://doi.org/10.1016/j.chembiol.2018.08.008
Publikationsstatus	Veröffentlicht - 20 Dez. 2018
Extern publiziert	Ja

Zugriff auf Dokument

10.1016/j.chembiol.2018.08.008

Andere Dateien und Links

Verknüpfung zur Publikation in Scopus

Dieses zitieren

@article{b806099851a74bd190461b191652f284,

title = "Imaging-Based Screening Platform Assists Protein Engineering",

abstract = "Protein evolution requires generation and screening of large numbers of variants. To expedite screening, Fabritius et al. built a platform that integrates fluorescence-based image analysis and picking of bacterial colonies. They engineered mCarmine, a far-red-emitting fluorescent protein for tagging and deep tissue imaging.",

keywords = "directed evolution, fluorescent proteins, image analysis, protein engineering, screening",

author = "Arne Fabritius and David Ng and Kist, {Andreas Michael} and Mutlu Erdogan and Ruben Portugues and Oliver Griesbeck",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2018",

month = dec,

day = "20",

doi = "10.1016/j.chembiol.2018.08.008",

language = "English",

volume = "25",

pages = "1554--1561.e8",

journal = "Cell Chemical Biology",

issn = "2451-9456",

publisher = "Elsevier Inc.",

number = "12",

}

TY - JOUR

T1 - Imaging-Based Screening Platform Assists Protein Engineering

AU - Fabritius, Arne

AU - Ng, David

AU - Kist, Andreas Michael

AU - Erdogan, Mutlu

AU - Portugues, Ruben

AU - Griesbeck, Oliver

PY - 2018/12/20

Y1 - 2018/12/20

N2 - Protein evolution requires generation and screening of large numbers of variants. To expedite screening, Fabritius et al. built a platform that integrates fluorescence-based image analysis and picking of bacterial colonies. They engineered mCarmine, a far-red-emitting fluorescent protein for tagging and deep tissue imaging.

AB - Protein evolution requires generation and screening of large numbers of variants. To expedite screening, Fabritius et al. built a platform that integrates fluorescence-based image analysis and picking of bacterial colonies. They engineered mCarmine, a far-red-emitting fluorescent protein for tagging and deep tissue imaging.

KW - directed evolution

KW - fluorescent proteins

KW - image analysis

KW - protein engineering

KW - screening

UR - http://www.scopus.com/inward/record.url?scp=85058908989&partnerID=8YFLogxK

U2 - 10.1016/j.chembiol.2018.08.008

DO - 10.1016/j.chembiol.2018.08.008

M3 - Article

C2 - 30220597

AN - SCOPUS:85058908989

SN - 2451-9456

VL - 25

SP - 1554-1561.e8

JO - Cell Chemical Biology

JF - Cell Chemical Biology

IS - 12

ER -

Imaging-Based Screening Platform Assists Protein Engineering

Abstract

Zugriff auf Dokument

Andere Dateien und Links

Fingerprint

Dieses zitieren