Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images

M. Athelogou; M. Eblenkamp; G. Schmidt; F. Novotny; E. Wintermantel; G. Binnig

doi:10.1007/978-3-642-19335-4_29

Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images

M. Athelogou, M. Eblenkamp, G. Schmidt, F. Novotny, E. Wintermantel, G. Binnig

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag › Begutachtung

Abstract

Because of the very special type of contrast in phase-contrast images, it is almost impossible to perform fully automated single-cell analysis and quantification successfully. Because fluorescent dyes are highly toxic, phase-contrast images are commonly used to monitor live cells. In this paper, we present a method for the fully automated segmentation, classification and quantification of individual cell morphology in phase-contrast images. We calculate the confluence of the cell population and quantify the degree of toxic damage to each individual cell following phenol incubation. The results are then compared to standard cytotoxicity assays.

Originalsprache	Englisch
Titel	Bildverarbeitung fur die Medizin 2011
Untertitel	Algorithmen - Systeme - Anwendungen, BVM 2011 - Proceedings des Workshops
Seiten	134-138
Seitenumfang	5
DOIs	https://doi.org/10.1007/978-3-642-19335-4_29
Publikationsstatus	Veröffentlicht - 2011
Veranstaltung	Workshops Bildverarbeitung fur die Medizin: Algorithmen - Systeme - Anwendungen, BVM 2011 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2011 - Lubeck, Deutschland Dauer: 20 März 2011 → 22 März 2011

Publikationsreihe

Name	Informatik aktuell
ISSN (Print)	1431-472X

Konferenz

Konferenz	Workshops Bildverarbeitung fur die Medizin: Algorithmen - Systeme - Anwendungen, BVM 2011 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2011
Land/Gebiet	Deutschland
Ort	Lubeck
Zeitraum	20/03/11 → 22/03/11

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10.1007/978-3-642-19335-4_29

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Athelogou, M., Eblenkamp, M., Schmidt, G., Novotny, F., Wintermantel, E., & Binnig, G. (2011). Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images. in Bildverarbeitung fur die Medizin 2011: Algorithmen - Systeme - Anwendungen, BVM 2011 - Proceedings des Workshops (S. 134-138). (Informatik aktuell). https://doi.org/10.1007/978-3-642-19335-4_29

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title = "Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images",

abstract = "Because of the very special type of contrast in phase-contrast images, it is almost impossible to perform fully automated single-cell analysis and quantification successfully. Because fluorescent dyes are highly toxic, phase-contrast images are commonly used to monitor live cells. In this paper, we present a method for the fully automated segmentation, classification and quantification of individual cell morphology in phase-contrast images. We calculate the confluence of the cell population and quantify the degree of toxic damage to each individual cell following phenol incubation. The results are then compared to standard cytotoxicity assays.",

author = "M. Athelogou and M. Eblenkamp and G. Schmidt and F. Novotny and E. Wintermantel and G. Binnig",

year = "2011",

doi = "10.1007/978-3-642-19335-4_29",

language = "English",

isbn = "9783642193347",

series = "Informatik aktuell",

pages = "134--138",

booktitle = "Bildverarbeitung fur die Medizin 2011",

note = "Workshops Bildverarbeitung fur die Medizin: Algorithmen - Systeme - Anwendungen, BVM 2011 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2011 ; Conference date: 20-03-2011 Through 22-03-2011",

}

Athelogou, M, Eblenkamp, M, Schmidt, G, Novotny, F, Wintermantel, E & Binnig, G 2011, Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images. in Bildverarbeitung fur die Medizin 2011: Algorithmen - Systeme - Anwendungen, BVM 2011 - Proceedings des Workshops. Informatik aktuell, S. 134-138, Workshops Bildverarbeitung fur die Medizin: Algorithmen - Systeme - Anwendungen, BVM 2011 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2011, Lubeck, Deutschland, 20/03/11. https://doi.org/10.1007/978-3-642-19335-4_29

Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images. / Athelogou, M.; Eblenkamp, M.; Schmidt, G. et al.
Bildverarbeitung fur die Medizin 2011: Algorithmen - Systeme - Anwendungen, BVM 2011 - Proceedings des Workshops. 2011. S. 134-138 (Informatik aktuell).

Publikation: Beitrag in Buch/Bericht/Konferenzband › Konferenzbeitrag › Begutachtung

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T1 - Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images

AU - Athelogou, M.

AU - Eblenkamp, M.

AU - Schmidt, G.

AU - Novotny, F.

AU - Wintermantel, E.

AU - Binnig, G.

PY - 2011

Y1 - 2011

N2 - Because of the very special type of contrast in phase-contrast images, it is almost impossible to perform fully automated single-cell analysis and quantification successfully. Because fluorescent dyes are highly toxic, phase-contrast images are commonly used to monitor live cells. In this paper, we present a method for the fully automated segmentation, classification and quantification of individual cell morphology in phase-contrast images. We calculate the confluence of the cell population and quantify the degree of toxic damage to each individual cell following phenol incubation. The results are then compared to standard cytotoxicity assays.

AB - Because of the very special type of contrast in phase-contrast images, it is almost impossible to perform fully automated single-cell analysis and quantification successfully. Because fluorescent dyes are highly toxic, phase-contrast images are commonly used to monitor live cells. In this paper, we present a method for the fully automated segmentation, classification and quantification of individual cell morphology in phase-contrast images. We calculate the confluence of the cell population and quantify the degree of toxic damage to each individual cell following phenol incubation. The results are then compared to standard cytotoxicity assays.

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U2 - 10.1007/978-3-642-19335-4_29

DO - 10.1007/978-3-642-19335-4_29

M3 - Conference contribution

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BT - Bildverarbeitung fur die Medizin 2011

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Y2 - 20 March 2011 through 22 March 2011

ER -

Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images

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