TY - JOUR
T1 - Human mesenchymal stem cells and renal tubular epithelial cells differentially influence monocyte-derived dendritic cell differentiation and maturation
AU - Kronsteiner, Barbara
AU - Peterbauer-Scherb, Anja
AU - Grillari-Voglauer, Regina
AU - Redl, Heinz
AU - Gabriel, Christian
AU - van Griensven, Martijn
AU - Wolbank, Susanne
PY - 2011
Y1 - 2011
N2 - Mesenchymal stem cells (MSCs) possess immunosuppressive properties. But also fully differentiated human renal tubular epithelial cells (RTECs) are able to modulate T-cell proliferation in vitro. In this study we compared two MSC populations, human adipose derived stem cells (ASCs) and human amniotic mesenchymal stromal cells (hAMSCs), and RTECs regarding their potential to inhibit monocyte-derived dendritic cell (DC) differentiation and maturation in indirect co-culture. In the presence of hAMSCs and RTECs, monocytes stimulated to undergo DC differentiation were inhibited to acquire surface phenotype of immature and mature DCs. In contrast, ASCs showed only limited suppressive capacity. Secretion of IL-12p70 was suppressed in hAMSC co-cultures and high IL-10 levels were detected in all co-cultures. Prostaglandin E2 was found in ASC and hAMSC co-cultures, whereas soluble human leukocyte antigen-G was highly elevated only in RTEC co-cultures. Thus, inhibition of DC generation by MSCs and RTECs might be mediated by different soluble factors.
AB - Mesenchymal stem cells (MSCs) possess immunosuppressive properties. But also fully differentiated human renal tubular epithelial cells (RTECs) are able to modulate T-cell proliferation in vitro. In this study we compared two MSC populations, human adipose derived stem cells (ASCs) and human amniotic mesenchymal stromal cells (hAMSCs), and RTECs regarding their potential to inhibit monocyte-derived dendritic cell (DC) differentiation and maturation in indirect co-culture. In the presence of hAMSCs and RTECs, monocytes stimulated to undergo DC differentiation were inhibited to acquire surface phenotype of immature and mature DCs. In contrast, ASCs showed only limited suppressive capacity. Secretion of IL-12p70 was suppressed in hAMSC co-cultures and high IL-10 levels were detected in all co-cultures. Prostaglandin E2 was found in ASC and hAMSC co-cultures, whereas soluble human leukocyte antigen-G was highly elevated only in RTEC co-cultures. Thus, inhibition of DC generation by MSCs and RTECs might be mediated by different soluble factors.
KW - Dendritic cells
KW - Immunomodulation
KW - Mesenchymal stem cells
KW - Renal tubular epithelial cells
UR - http://www.scopus.com/inward/record.url?scp=78651060858&partnerID=8YFLogxK
U2 - 10.1016/j.cellimm.2010.11.001
DO - 10.1016/j.cellimm.2010.11.001
M3 - Article
C2 - 21145536
AN - SCOPUS:78651060858
SN - 0008-8749
VL - 267
SP - 30
EP - 38
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -