TY - GEN
T1 - High-resolution tomographic imaging of microvessels
AU - Müller, Bert
AU - Lang, Sabrina
AU - Dominietto, Marco
AU - Rudin, Markus
AU - Schulz, Georg
AU - Deyhle, Hans
AU - Germann, Marco
AU - Pfeiffer, Franz
AU - David, Christian
AU - Weitkamp, Timm
PY - 2008
Y1 - 2008
N2 - Cancer belongs to the primary diseases these days. Although different successful treatments including surgery, chemical, pharmacological, and radiation therapies are established, the aggressive proliferation of cancerous cells and the related formation of blood vessels has to be better understood to develop more powerful strategies against the different kinds of cancer. Angiogenesis is one of the crucial steps for the survival and metastasis formation of malignant tumors. Although therapeutic strategies attempting to inhibit these processes are being developed, the biological regulation is still unclear. This study concentrates on the three-dimensional morphology of vessels formed in a mouse tumor xenograft model post mortem. Synchrotron radiation-based micro computed tomography (SRμCT) could provide the necessary information that is essential for validating the simulations. Using mouse and human brain tissue, the different approaches to extract the vessel tree from SRμCT data are discussed. These approaches include corrosion casting, the application of contrast agents such as barium sulfate, tissue embedding, all of them regarded as materials science based. Alternatively, phase contrast tomography was used, which gave rise to promising results but still not reaches the spatial resolution to uncover the smallest capillaries.
AB - Cancer belongs to the primary diseases these days. Although different successful treatments including surgery, chemical, pharmacological, and radiation therapies are established, the aggressive proliferation of cancerous cells and the related formation of blood vessels has to be better understood to develop more powerful strategies against the different kinds of cancer. Angiogenesis is one of the crucial steps for the survival and metastasis formation of malignant tumors. Although therapeutic strategies attempting to inhibit these processes are being developed, the biological regulation is still unclear. This study concentrates on the three-dimensional morphology of vessels formed in a mouse tumor xenograft model post mortem. Synchrotron radiation-based micro computed tomography (SRμCT) could provide the necessary information that is essential for validating the simulations. Using mouse and human brain tissue, the different approaches to extract the vessel tree from SRμCT data are discussed. These approaches include corrosion casting, the application of contrast agents such as barium sulfate, tissue embedding, all of them regarded as materials science based. Alternatively, phase contrast tomography was used, which gave rise to promising results but still not reaches the spatial resolution to uncover the smallest capillaries.
KW - Blood vessel staining
KW - Micro computed tomography
KW - Microtomography
KW - Post mortem imaging
KW - Tumor growth modeling
KW - Vessel formation
KW - X-ray phase contrast
UR - http://www.scopus.com/inward/record.url?scp=56249142747&partnerID=8YFLogxK
U2 - 10.1117/12.794157
DO - 10.1117/12.794157
M3 - Conference contribution
AN - SCOPUS:56249142747
SN - 9780819472984
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Developments in X-Ray Tomography VI
T2 - Developments in X-Ray Tomography VI
Y2 - 12 August 2008 through 14 August 2008
ER -