High efficiency of a new immunological magnetic cell sorting method for T cell depletion of human bone marrow

G. Kogler, A. B. Capdeville, M. Hauch, H. T. Bruster, U. Gobel, P. Wernet, S. Burdach

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

9 Zitate (Scopus)

Abstract

Fresh human bone marrow (BM) mononuclear cells containing a mean of 11% T cells underwent T cell depletion utilizing a newly developed magnetic cell sorter. In order to remove T cells, 108 cells were labeled with either a specific CD3 monoclonal antibody or a cocktail of monoclonal CD2+CD5+CD7 antibodies and biotin-conjugated magnetic microparticles (size 100-150 nm) employing a streptavidin-FITC complex. Labeled cells were removed by using columns filled with a ferromagnetic matrix and placed within a recess in a strong permanent magnet. Recovery of non-T cells was 79% after the first passage and 61% after the second. The absolute recovery of BFU-E and CFU-GM was 70% and 51% respectively (n=11). Limiting dilution analysis revealed less than 0.1% residual clonable BM T cells (cloning efficiency >50%) which corresponds to a 2.1 (1st passage) and to a 2,3 (2nd passage) log depletion. This new separating system is a highly efficient, reproducible, and quick method of depleting T cells from human BM. It could be easily adapted to process large scale marrow specimens or for the elimination of residual tumor cells from BM in autologous transplantation.

OriginalspracheEnglisch
Seiten (von - bis)163-168
Seitenumfang6
FachzeitschriftBone Marrow Transplantation
Jahrgang6
Ausgabenummer3
PublikationsstatusVeröffentlicht - 1990
Extern publiziertJa

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