TY - JOUR
T1 - Genetic Variation for Cold Tolerance in Two Nested Association Mapping Populations
AU - Revilla, Pedro
AU - Butrón, Ana
AU - Rodriguez, Víctor Manuel
AU - Rincent, Renaud
AU - Charcosset, Alain
AU - Giauffret, Catherine
AU - Melchinger, Albrecht E.
AU - Schön, Chris Carolin
AU - Bauer, Eva
AU - Altmann, Thomas
AU - Brunel, Dominique
AU - Moreno-González, Jesús
AU - Campo, Laura
AU - Ouzunova, Milena
AU - Álvarez, Ángel
AU - Ruíz de Galarreta, José Ignacio
AU - Laborde, Jacques
AU - Malvar, Rosa Ana
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/1
Y1 - 2023/1
N2 - Cold reduces maize (Zea mays L.) production and delays sowings. Cold tolerance in maize is very limited, and breeding maize for cold tolerance is still a major challenge. Our objective was to detect QTL for cold tolerance at germination and seedling stages. We evaluated, under cold and control conditions, 919 Dent and 1009 Flint inbred lines from two nested association mapping designs consisting in 24 double-haploid populations, genotyped with 56,110 SNPs. We found a large diversity of maize cold tolerance within these NAM populations. We detected one QTL for plant weight and four for fluorescence under cold conditions, as well as one for plant weight and two for chlorophyll content under control conditions in the Dent-NAM. There were fewer significant QTL under control conditions than under cold conditions, and half of the QTL were for quantum efficiency of photosystem II. Our results supported the large genetic discrepancy between optimal and low temperatures, as the quantity and the position of the QTL were very variable between control and cold conditions. Furthermore, as we have not found alleles with significant effects on these NAM designs, further studies are needed with other experimental designs to find favorable alleles with important effects for improving cold tolerance in maize.
AB - Cold reduces maize (Zea mays L.) production and delays sowings. Cold tolerance in maize is very limited, and breeding maize for cold tolerance is still a major challenge. Our objective was to detect QTL for cold tolerance at germination and seedling stages. We evaluated, under cold and control conditions, 919 Dent and 1009 Flint inbred lines from two nested association mapping designs consisting in 24 double-haploid populations, genotyped with 56,110 SNPs. We found a large diversity of maize cold tolerance within these NAM populations. We detected one QTL for plant weight and four for fluorescence under cold conditions, as well as one for plant weight and two for chlorophyll content under control conditions in the Dent-NAM. There were fewer significant QTL under control conditions than under cold conditions, and half of the QTL were for quantum efficiency of photosystem II. Our results supported the large genetic discrepancy between optimal and low temperatures, as the quantity and the position of the QTL were very variable between control and cold conditions. Furthermore, as we have not found alleles with significant effects on these NAM designs, further studies are needed with other experimental designs to find favorable alleles with important effects for improving cold tolerance in maize.
KW - NAM
KW - QTL
KW - RIL
KW - cold tolerance
KW - maize
UR - http://www.scopus.com/inward/record.url?scp=85146775092&partnerID=8YFLogxK
U2 - 10.3390/agronomy13010195
DO - 10.3390/agronomy13010195
M3 - Article
AN - SCOPUS:85146775092
SN - 2073-4395
VL - 13
JO - Agronomy
JF - Agronomy
IS - 1
M1 - 195
ER -