Generation of targeted mouse mutants by embryo microinjection of TALENs

Benedikt Wefers; Oskar Ortiz; Wolfgang Wurst; Ralf Kühn

doi:10.1016/j.ymeth.2014.01.002

Generation of targeted mouse mutants by embryo microinjection of TALENs

Benedikt Wefers, Oskar Ortiz, Wolfgang Wurst, Ralf Kühn

Lehrstuhl für Entwicklungsgenetik

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

14 Zitate (Scopus)

Abstract

Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.

Originalsprache	Englisch
Seiten (von - bis)	94-101
Seitenumfang	8
Fachzeitschrift	Methods
Jahrgang	69
Ausgabenummer	1
DOIs	https://doi.org/10.1016/j.ymeth.2014.01.002
Publikationsstatus	Veröffentlicht - 2014

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10.1016/j.ymeth.2014.01.002

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abstract = "Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.",

keywords = "Gene targeting, Knockin, Knockout, Mouse, One-cell embryo, TALEN",

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doi = "10.1016/j.ymeth.2014.01.002",

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AU - Wefers, Benedikt

AU - Ortiz, Oskar

AU - Wurst, Wolfgang

AU - Kühn, Ralf

PY - 2014

Y1 - 2014

N2 - Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.

AB - Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step, without the need for embryonic stem cells. Thereby, knockout and knockin alleles can be generated fast and efficiently by embryo microinjection of TALEN mRNAs and targeting vectors. In this article we present an introduction into the TALEN technology and provide protocols for the application of TALENs in mouse zygotes.

KW - Gene targeting

KW - Knockin

KW - Knockout

KW - Mouse

KW - One-cell embryo

KW - TALEN

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SN - 1046-2023

VL - 69

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JO - Methods

JF - Methods

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ER -

Generation of targeted mouse mutants by embryo microinjection of TALENs

Abstract

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