Flattop regulates basal body docking and positioning in mono- and multiciliated cells

Moritz Gegg, Anika Böttcher, Ingo Burtscher, Stefan Hasenoeder, Claude Van Campenhout, Michaela Aichler, Axel Walch, Seth G.N. Grant, Heiko Lickert

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

42 Zitate (Scopus)

Abstract

Planar cell polarity (PCP) regulates basal body (BB) docking and positioning during cilia formation, but the underlying mechanisms remain elusive. In this study, we investigate the uncharacterized gene Flattop (Fltp) that is transcriptionally activated during PCP acquisition in ciliated tissues. Fltp knock-out mice show BB docking and ciliogenesis defects in multiciliated lung cells. Furthermore, Fltp is necessary for kinocilium positioning in monociliated inner ear hair cells. In these cells, the core PCP molecule Dishevelled 2, the BB/spindle positioning protein Dlg3, and Fltp localize directly adjacent to the apical plasma membrane, physically interact and surround the BB at the interface of the microtubule and actin cytoskeleton. Dlg3 and Fltp knock-outs suggest that both cooperatively translate PCP cues for BB positioning in the inner ear. Taken together, the identification of novel BB/spindle positioning components as potential mediators of PCP signaling might have broader implications for other cell types, ciliary disease, and asymmetric cell division.

OriginalspracheEnglisch
Aufsatznummere03842
FachzeitschrifteLife
Jahrgang3
DOIs
PublikationsstatusVeröffentlicht - 8 Okt. 2014
Extern publiziertJa

Fingerprint

Untersuchen Sie die Forschungsthemen von „Flattop regulates basal body docking and positioning in mono- and multiciliated cells“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren