@article{bf301771443b485d9b40774429e36b75,
title = "Fed-batch production of a soluble β-1,4-galactosyltransferase with Saccharomyces cerevisiae",
abstract = "A feedback-controlled fed-batch process for the recombinant production of a soluble human β-1,4-galactosyltransferase (NdrGal-T) with Saccharomyces cerevisiae was developed and scaled up to the pilot scale. A 5000 U NdrGal-T fermentation run was performed on a 300 l scale. Indirect feedback control of the glucose feeding with RQ data at a set-point of RQ = 1.1 resulted in higher NdrGal-T activities (30 U/l) than direct feedback control of glucose with on-line flow-injection analysis (9 U/l). This increase in final activity of NdrGal-T by a factor of 42 compared to published data makes the fed-batch production of glycosyltransferases with a Saccharomyces cerevisiae expression system competitive with cell-culture systems. Copyright (C) 1999 Elsevier Science Inc.",
keywords = "Feedback control, Flow-injection analysis, Glycosyltransferase, Kalman filter, Respiratory quotient (RQ), Saccharomyces cerevisiae",
author = "Jana Zigovaa and Matthias Mahlea and Holger Pascholda and Martine Malissard and Berger, {Eric G.} and Dirk Weuster-Botz",
note = "Funding Information: The authors are deeply indebted to Prof. Dr. C. Wandrey, Research Centre Juelich, Institute of Biotechnology, Germany for the excellent support of this work and for the possibility of using the extensive technical facilities of the Institute of Biotechnology. The authors are grateful to the NOVARTIS company, Basel, Switzerland, for supplying the Saccharomyces cerevisiae strain BT150 deficient in proteases. The grant from the Hermann–Schlosser Foundation, Frankfurt, Germany, for supporting the stay of J. Zigova at the Research Centre Juelich is gratefully acknowledged. Part of this work was supported by Grant 5002-46084 from the Swiss National Science Foundation.",
year = "1999",
month = aug,
doi = "10.1016/S0141-0229(99)00027-7",
language = "English",
volume = "25",
pages = "201--207",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier Inc.",
number = "3-5",
}