Expression of the hepatitis B surface antigen (HbsAg) under the control of mMT-I promoter in transgenic mice can be induced by zinc sulphate, dexamethasone and lipopolysaccharide

Metallothionein genes can be induced in vivo by heavy metals, glucocorticoids, and toxins. In all transgenic mice carrying the MT-I promoter, that have been reported so far, induction by glucocorticoids failed. This study reports two mouse lines, transgenic for the murine MT-I-HBV (hepatitis B virus; map position site: 30-1986) construct, which secrete the viral surface antigen (HBsAg) in their serum. In both lines, males produce more HBsAg than females, and in all cases the MT-I promoter can be induced by dexamethasone, lipopolysaccharide (LPS), and heavy metals. A glucocorticoid-responsive element, which is situated in the HBV fragment used, can explain the dexamethasone induction of the MT-I promoter.