TY - JOUR
T1 - Escherichia coli DNA synthesis in vitro
T2 - Insensitivity of ATP-dependent DNA repair to inhibition by novobiocin
AU - Schneck, Peter K.
AU - Staudenbauer, Walter L.
N1 - Funding Information:
Fig. 3: Pycnographic analysis in alkaline CsCl of extensively sheared repaired DNA UV-irradiated toluenized cells (0.4 ml) were incubated in a standard incubation mixture (total volume 1.0 ml) containing dBrUTP for 30 min at 44°C in the absence (A) or presence (B) of novobiocin (100 g/ml). Repaired DNA of parental density was isolated as described in Fig. 2 and extensively sheared by 2 min of sonication with a Branson Instruments sonifier at maximum power setting (16). The extent of shear was estimated from the sedimentation coefficient of the DNA fragments determined by alkaline velocity sedimentation (18). The sheared DNA was centrifuged to equilibrium in a CsCl gradient containing 0.2 N NaOH (13). Density increases from right to left. (• • ) , 32P-labeled repaired DNA; (o o) H-prelabeled DNA This work was supported by grant Sta 136/3 from the Deutsche Forschungsgemeinschaft. We thank Dr. P. H. Hofschneider for critical reading of the manuscript and helpful comments.
PY - 1977/6
Y1 - 1977/6
N2 - Novobiocin, an effective inhibitor of DNA replication in Escherichia coli, is shown to have no effect on the ATP-dependent DNA repair carried out by toluenized cells after ultraviolet irradiation. Therefore novobiocin can be considered a selective inhibitor of replicative DNA synthesis in vitro.
AB - Novobiocin, an effective inhibitor of DNA replication in Escherichia coli, is shown to have no effect on the ATP-dependent DNA repair carried out by toluenized cells after ultraviolet irradiation. Therefore novobiocin can be considered a selective inhibitor of replicative DNA synthesis in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0017682811&partnerID=8YFLogxK
U2 - 10.1093/nar/4.6.2057
DO - 10.1093/nar/4.6.2057
M3 - Article
C2 - 197497
AN - SCOPUS:0017682811
SN - 0305-1048
VL - 4
SP - 2057
EP - 2064
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 6
ER -