TY - JOUR
T1 - Enzymes of the glutathione-ascorbate cycle in leaves and roots of rhizobia-inoculated faba bean plants (Vicia faba L.) under salinity stress
AU - Oufdou, Khalid
AU - Benidire, Loubna
AU - Lyubenova, Lyudmila
AU - Daoui, Khalid
AU - El Abidine Fatemi, Zain
AU - Schröder, Peter
N1 - Funding Information:
The research stay of Khalid OUFDOU in Helmholtz Zentrum, München is financially supported by the DEUTSCHER AKADEMISCHER AUSTAUSCHDIENST (DAAD) . The authors thank to R. Harpaintner and S. Daro for their help and technical assistance and T. Smith for professional language editing of the manuscript. This work is a part of the project PRAD 11-01-Egide N° 24186QM.
PY - 2014/1
Y1 - 2014/1
N2 - Grain legumes such as the faba bean (Vicia faba L.) used for human as well as animal nutrition, are of great importance especially in countries under Mediterranean climate like Morocco, which is mainly characterized by semi-arid and arid climates and by saline soils. The objective of the present study was to evaluate the effects of salt stress on growth, nodulation and the enzymes of the glutathione-ascorbate cycle of faba bean plants (Moroccan cultivar "Aguadulce"). The experiments were carried out under greenhouse conditions, where faba bean plants were grown under control (nutrient solution) or salt stress conditions (nutrient solution containing 75mM or 150mM of NaCl). The activity of enzymes of the glutathione-ascorbate cycle was studied in leaves and roots of faba bean plants inoculated by rhizobia RhOF4 or RhOF6, isolated from nodules of faba bean cultures in the region of Marrakech. The growth of faba bean plants decreased with increasing salt concentrations. Total weight and length of shoots significantly decreased under NaCl stress (p<0.05), especially at 150mM. Also the nodulation of the roots was strongly decreased under salinity stress, with reductions of 91.35% and of 88.67% in the total nodule number of plants exposed to 150mM of NaCl and inoculated by RhOF4 and by RhOF6 respectively. NaCl effects on the glutathione-ascorbate cycle were more pronounced in roots inoculated by RhOF6. Stress responding glutathione S-transferase (GST) activity was generally increased in roots inoculated by RhOF6 and submitted to salt stress. On the contrary, specific activities of glutathione peroxidase (GPOX), superoxide dismutase (SOD), ascorbate peroxidase (APOX) and monodehydroascorbate reductase (MDHAR) were reduced in roots of faba bean plants inoculated by RhOF6 exposed to saline treatment as compared to the controls. Our results show the importance of several enzymes of the ascorbate-glutathione cycle and the reduced glutathione (GSH) during some faba bean-rhizobia symbiotic combinations, in root defence and adaptation against salt stress conditions.
AB - Grain legumes such as the faba bean (Vicia faba L.) used for human as well as animal nutrition, are of great importance especially in countries under Mediterranean climate like Morocco, which is mainly characterized by semi-arid and arid climates and by saline soils. The objective of the present study was to evaluate the effects of salt stress on growth, nodulation and the enzymes of the glutathione-ascorbate cycle of faba bean plants (Moroccan cultivar "Aguadulce"). The experiments were carried out under greenhouse conditions, where faba bean plants were grown under control (nutrient solution) or salt stress conditions (nutrient solution containing 75mM or 150mM of NaCl). The activity of enzymes of the glutathione-ascorbate cycle was studied in leaves and roots of faba bean plants inoculated by rhizobia RhOF4 or RhOF6, isolated from nodules of faba bean cultures in the region of Marrakech. The growth of faba bean plants decreased with increasing salt concentrations. Total weight and length of shoots significantly decreased under NaCl stress (p<0.05), especially at 150mM. Also the nodulation of the roots was strongly decreased under salinity stress, with reductions of 91.35% and of 88.67% in the total nodule number of plants exposed to 150mM of NaCl and inoculated by RhOF4 and by RhOF6 respectively. NaCl effects on the glutathione-ascorbate cycle were more pronounced in roots inoculated by RhOF6. Stress responding glutathione S-transferase (GST) activity was generally increased in roots inoculated by RhOF6 and submitted to salt stress. On the contrary, specific activities of glutathione peroxidase (GPOX), superoxide dismutase (SOD), ascorbate peroxidase (APOX) and monodehydroascorbate reductase (MDHAR) were reduced in roots of faba bean plants inoculated by RhOF6 exposed to saline treatment as compared to the controls. Our results show the importance of several enzymes of the ascorbate-glutathione cycle and the reduced glutathione (GSH) during some faba bean-rhizobia symbiotic combinations, in root defence and adaptation against salt stress conditions.
KW - Antioxidative enzymes
KW - Glutathione pathway
KW - Rhizobia
KW - Salt stress
KW - Symbiosis
KW - Vicia faba L.
UR - http://www.scopus.com/inward/record.url?scp=84890812076&partnerID=8YFLogxK
U2 - 10.1016/j.ejsobi.2013.11.002
DO - 10.1016/j.ejsobi.2013.11.002
M3 - Article
AN - SCOPUS:84890812076
SN - 1164-5563
VL - 60
SP - 98
EP - 103
JO - European Journal of Soil Biology
JF - European Journal of Soil Biology
ER -