Abstract
The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as efficiently as intact PE2 in human cells. We use this Split-PE system to rapidly identify and engineer more compact prime editor architectures that also broaden the types of RTs used for prime editing.
Originalsprache | Englisch |
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Seiten (von - bis) | 337-343 |
Seitenumfang | 7 |
Fachzeitschrift | Nature Biotechnology |
Jahrgang | 41 |
Ausgabenummer | 3 |
DOIs | |
Publikationsstatus | Veröffentlicht - März 2023 |