Electrochemical microfluidic biosensor for the protein-based quantification of β-lactams

In this work, we present a novel approach for the point-of-care testing (POCT) of β-lactam antibiotics on a miniaturized electrochemical microfluidic platform. Herein, a highly sensitive, lowcost and versatile biosensor is combined with an enzyme linked assay (ELA) using the penicillin binding protein 3 (PBP-3) for the fast and facile detection of s-lactams from very low sample volumes (580 nl). The demonstration of the system applicability is successfully shown by measuring different piperacillin concentrations in spiked human blood plasma with an inter-assay coefficient of variation (CV) below 15 % and a limit of detection (LOD) of 8.34 ng ml^-1.