TY - JOUR
T1 - Eigenspectra optoacoustic tomography achieves quantitative blood oxygenation imaging deep in tissues
AU - Tzoumas, Stratis
AU - Nunes, Antonio
AU - Olefir, Ivan
AU - Stangl, Stefan
AU - Symvoulidis, Panagiotis
AU - Glasl, Sarah
AU - Bayer, Christine
AU - Multhoff, Gabriele
AU - Ntziachristos, Vasilis
PY - 2016/6/30
Y1 - 2016/6/30
N2 - Light propagating in tissue attains a spectrum that varies with location due to wavelength-dependent fluence attenuation, an effect that causes spectral corruption. Spectral corruption has limited the quantification accuracy of optical and optoacoustic spectroscopic methods, and impeded the goal of imaging blood oxygen saturation (sO 2) deep in tissues; a critical goal for the assessment of oxygenation in physiological processes and disease. Here we describe light fluence in the spectral domain and introduce eigenspectra multispectral optoacoustic tomography (eMSOT) to account for wavelength-dependent light attenuation, and estimate blood sO 2 within deep tissue. We validate eMSOT in simulations, phantoms and animal measurements and spatially resolve sO 2 in muscle and tumours, validating our measurements with histology data. eMSOT shows substantial sO 2 accuracy enhancement over previous optoacoustic methods, potentially serving as a valuable tool for imaging tissue pathophysiology.
AB - Light propagating in tissue attains a spectrum that varies with location due to wavelength-dependent fluence attenuation, an effect that causes spectral corruption. Spectral corruption has limited the quantification accuracy of optical and optoacoustic spectroscopic methods, and impeded the goal of imaging blood oxygen saturation (sO 2) deep in tissues; a critical goal for the assessment of oxygenation in physiological processes and disease. Here we describe light fluence in the spectral domain and introduce eigenspectra multispectral optoacoustic tomography (eMSOT) to account for wavelength-dependent light attenuation, and estimate blood sO 2 within deep tissue. We validate eMSOT in simulations, phantoms and animal measurements and spatially resolve sO 2 in muscle and tumours, validating our measurements with histology data. eMSOT shows substantial sO 2 accuracy enhancement over previous optoacoustic methods, potentially serving as a valuable tool for imaging tissue pathophysiology.
UR - http://www.scopus.com/inward/record.url?scp=84977161109&partnerID=8YFLogxK
U2 - 10.1038/ncomms12121
DO - 10.1038/ncomms12121
M3 - Article
C2 - 27358000
AN - SCOPUS:84977161109
SN - 2041-1723
VL - 7
JO - Nature Communications
JF - Nature Communications
M1 - 12121
ER -