TY - JOUR
T1 - Effects of Light on Growth and Metabolism of Rhodococcus erythropolis
AU - Engelhart-Straub, Selina
AU - Cavelius, Philipp
AU - Hölzl, Fabian
AU - Haack, Martina
AU - Awad, Dania
AU - Brueck, Thomas
AU - Mehlmer, Norbert
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/8
Y1 - 2022/8
N2 - Rhodococcus erythropolis is resilient to various stressors. However, the response of R. erythropolis towards light has not been evaluated. In this study, R. erythropolis was exposed to different wavelengths of light. Compared to non-illuminated controls, carotenoid levels were significantly increased in white (standard warm white), green (510 nm) and blue light (470 nm) illuminated cultures. Notably, blue light (455, 425 nm) exhibited anti-microbial effects. Interestingly, cellular lipid composition shifted under light stress, increasing odd chain fatty acids (C15:0, C17:1) cultured under white (standard warm white) and green (510 nm) light. When exposed to blue light (470, 455, 425 nm), fatty acid profiles shifted to more saturated fatty acids (C16:1 to C16:0). Time-resolved proteomics analysis revealed several oxidative stress-related proteins to be upregulated under light illumination.
AB - Rhodococcus erythropolis is resilient to various stressors. However, the response of R. erythropolis towards light has not been evaluated. In this study, R. erythropolis was exposed to different wavelengths of light. Compared to non-illuminated controls, carotenoid levels were significantly increased in white (standard warm white), green (510 nm) and blue light (470 nm) illuminated cultures. Notably, blue light (455, 425 nm) exhibited anti-microbial effects. Interestingly, cellular lipid composition shifted under light stress, increasing odd chain fatty acids (C15:0, C17:1) cultured under white (standard warm white) and green (510 nm) light. When exposed to blue light (470, 455, 425 nm), fatty acid profiles shifted to more saturated fatty acids (C16:1 to C16:0). Time-resolved proteomics analysis revealed several oxidative stress-related proteins to be upregulated under light illumination.
KW - Rhodococcus
KW - antimicrobial blue light
KW - carotenoids
KW - fatty acids
KW - photo-oxidative stress
KW - stress response
KW - time-resolved proteomics
UR - http://www.scopus.com/inward/record.url?scp=85137343545&partnerID=8YFLogxK
U2 - 10.3390/microorganisms10081680
DO - 10.3390/microorganisms10081680
M3 - Article
AN - SCOPUS:85137343545
SN - 2076-2607
VL - 10
JO - Microorganisms
JF - Microorganisms
IS - 8
M1 - 1680
ER -