TY - JOUR
T1 - Effect of prohexadione-Ca on various fruit crops
T2 - Flavonoid composition and substrate specificity of their dihydroflavonol 4-reductases
AU - Gosch, C.
AU - Puhl, I.
AU - Halbwirth, H.
AU - Schlangen, K.
AU - Roemmelt, S.
AU - Andreotti, C.
AU - Costa, G.
AU - Fischer, T. C.
AU - Treutter, D.
AU - Stich, K.
AU - Forkmann, G.
PY - 2003/5
Y1 - 2003/5
N2 - In pome fruit trees, the growth retardant prohexadione-Ca induces the formation of 3-deoxycatechins, which correlates with an increased resistance against fire blight and also against some fungal diseases. The formation of 3-deoxycatechins is due to the channelling of intermediates unusual for pome fruits, which requires the presence of a flavanone 4-reductase (FNR) activity. Here, it is investigated, whether the crop plants strawberry, grapevine, cranberry, cherry, peach, plum, elder, and kiwi fruit react in the same way to prohexadione-Ca application. The presence of FNR activity is shown by enzymatic investigations and the formation of a 3-deoxycatechin after prohexadione-Ca application in planta. Furthermore, by cloning of the corresponding cDNAs and studies with the heterologously expressed enzymes it is shown that the DFR enzymes of grapevine, cranberry and strawberry posses also FNR activity. Conclusively, all the crop plants studied have the potential to form 3-deoxyflavonoids. However, the changes in the flavonoid composition observed after prohexadione-Ca treatment were not exactly the same in all species studied here. In kiwi fruit leaves, a strong accumulation of flavanones rather than the formation of large amounts of 3-deoxycatechins is observed.
AB - In pome fruit trees, the growth retardant prohexadione-Ca induces the formation of 3-deoxycatechins, which correlates with an increased resistance against fire blight and also against some fungal diseases. The formation of 3-deoxycatechins is due to the channelling of intermediates unusual for pome fruits, which requires the presence of a flavanone 4-reductase (FNR) activity. Here, it is investigated, whether the crop plants strawberry, grapevine, cranberry, cherry, peach, plum, elder, and kiwi fruit react in the same way to prohexadione-Ca application. The presence of FNR activity is shown by enzymatic investigations and the formation of a 3-deoxycatechin after prohexadione-Ca application in planta. Furthermore, by cloning of the corresponding cDNAs and studies with the heterologously expressed enzymes it is shown that the DFR enzymes of grapevine, cranberry and strawberry posses also FNR activity. Conclusively, all the crop plants studied have the potential to form 3-deoxyflavonoids. However, the changes in the flavonoid composition observed after prohexadione-Ca treatment were not exactly the same in all species studied here. In kiwi fruit leaves, a strong accumulation of flavanones rather than the formation of large amounts of 3-deoxycatechins is observed.
KW - 3-deoxyflavonoids
KW - Actinidia sp. (kiwi fruit)
KW - Flavanone 4-reductase (FNR)
KW - Fragaria x ananassa (strawberry)
KW - Induced resistance
KW - Prunus domestica (plum)
KW - Prunus persica (peach)
KW - Vitis vinifera (grapevine)
UR - http://www.scopus.com/inward/record.url?scp=0141453157&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0141453157
SN - 1611-4434
VL - 68
SP - 144
EP - 151
JO - European Journal of Horticultural Science
JF - European Journal of Horticultural Science
IS - 3
ER -