Effect of different γ-irradiation on cytotoxicity and material properties of porous polyether-urethane polymer

H. J. Haugen, M. Brunner, F. Pellkofer, J. Aigner, J. Will, E. Wintermantel

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

31 Zitate (Scopus)

Abstract

Biomaterials respond to sterilization methods differently. Steam sterilization might decrease the performance of thermoplastic polyether-urethane (TPU); however, the effect of different γ-radiation doses on this polymer is contradictory in present literature. The purpose of this study was to investigate the differences between irradiative doses in comparison with steam sterilization on a porous TPU scaffold produced by a new processing method. No significant differences in the surface chemical structure were found with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) analysis when comparing with the sterilization methods. The molecular weight (Mw) had a net increase from 11.5 ± 0.039 to 13.2 ± 0.072 kDa by γ-sterilization from 10 to 60 kGy. The samples that were irradiated (>60 kGy) had also an increase in polydispersity index (PDI; 1.45 ± 0.007) in comparison with the nonsterile ones (1.31 ± 0.017), which indicate branching. Liquid chromatography/mass spectroscopy (LC/MS) analysis showed that there was a correlation between the concentration of the breakdown product, methyl dianiline, and cytotoxicity. The concentration of this compound was found to be four times higher in steam-sterilized sample (1.3 ± 0.01 ppb) compared with that of the polymer sample γ-sterilized at 10 kGy (0.3 ± 0.01 ppb). The cytotoxicity of TPU was found to decrease with higher radiation doses, and was significantly higher for the steam-sterilized samples. It is recommended that TPU produced with the described foaming method should be sterilized by γ-irradiation at 25 kGy or higher doses.

OriginalspracheEnglisch
Seiten (von - bis)415-423
Seitenumfang9
FachzeitschriftJournal of Biomedical Materials Research - Part B Applied Biomaterials
Jahrgang80
Ausgabenummer2
DOIs
PublikationsstatusVeröffentlicht - Feb. 2007

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