Direct comparison of 3D and 2D cultivation reveals higher osteogenic capacity of elderly osteoblasts in 3D

Stephan Payr, Elizabeth Rosado-Balmayor, Thomas Tiefenboeck, Tim Schuseil, Marina Unger, Claudine Seeliger, Martijn van Griensven

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

10 Zitate (Scopus)


Background: The aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture. Methods: Osteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression. Results: Osteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002). Conclusion: Advanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people. Trial registration: 5217/11 on the 22nd of Dec. 2011.

FachzeitschriftJournal of Orthopaedic Surgery and Research
PublikationsstatusVeröffentlicht - Dez. 2021
Extern publiziertJa


Untersuchen Sie die Forschungsthemen von „Direct comparison of 3D and 2D cultivation reveals higher osteogenic capacity of elderly osteoblasts in 3D“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren