TY - JOUR
T1 - Different enzymes involved in NADH- and NADPH-dependent respiration in the cyanobacterium Anabaena variabilis
AU - Alpes, I.
AU - Schrautemeier, B.
AU - Scherer, S.
AU - Böger, P.
N1 - Funding Information:
This study was supported by the Deutsche For-schungsgemeinschaft (grant Bo 310/12-4).
PY - 1985/2
Y1 - 1985/2
N2 - Filaments of N2-grown Anabaena variabilis exhibit soluble NADPH- and membrane-bound NADH-oxidizing activities. The NADPH-specific enzyme has been identified as ferredoxin-NADP oxidoreductase (FNR; EC 1.18.1.2) by the thionicotinamide-NADP transhydrogenase test, a ferredoxin-dependent hydrogenase assay, and by diaphorase systems. The FNR is easily removed by washing of French-press-prepared membranes. Concurrently, a loss of NADPH-dependent respiration is apparent, which is not reconstitutable by addition of Anabaena cytochrome c-553. The NADH-oxidizing activity, however, is only slightly affected by the washing procedure, and is completely reconstituted by cytochrome c-553. NADPH-dependent oxygen uptake is strongly inhibited by NADP, whereas inhibition of NADH-dependent oxygen uptake by NAD is less pronounced. The data give evidence that NADH and NADPH oxidations linked to the respiratory chain are mediated by two different enzymes.
AB - Filaments of N2-grown Anabaena variabilis exhibit soluble NADPH- and membrane-bound NADH-oxidizing activities. The NADPH-specific enzyme has been identified as ferredoxin-NADP oxidoreductase (FNR; EC 1.18.1.2) by the thionicotinamide-NADP transhydrogenase test, a ferredoxin-dependent hydrogenase assay, and by diaphorase systems. The FNR is easily removed by washing of French-press-prepared membranes. Concurrently, a loss of NADPH-dependent respiration is apparent, which is not reconstitutable by addition of Anabaena cytochrome c-553. The NADH-oxidizing activity, however, is only slightly affected by the washing procedure, and is completely reconstituted by cytochrome c-553. NADPH-dependent oxygen uptake is strongly inhibited by NADP, whereas inhibition of NADH-dependent oxygen uptake by NAD is less pronounced. The data give evidence that NADH and NADPH oxidations linked to the respiratory chain are mediated by two different enzymes.
KW - (NAD(P)H oxidation
KW - NADH dehydrogenase
KW - blue-green algae)
KW - ferredoxin-NADP oxidoreductase
UR - http://www.scopus.com/inward/record.url?scp=84982626308&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1985.tb01582.x
DO - 10.1111/j.1574-6968.1985.tb01582.x
M3 - Article
AN - SCOPUS:84982626308
SN - 0378-1097
VL - 26
SP - 147
EP - 151
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -