Development of Enzyme Immunoassays for the Detection of Pyrethroid Insecticides 2. Polyclonal Antibodies for Pyrethroid Insecticides

Sabine Pullen, Bertold Hock

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

12 Zitate (Scopus)

Abstract

An enzyme immunoassay was developed for the simultaneous detection of different pyrethroid insecticides. Several polyclonal antisera were raised in rabbits using 1R trans-permethric acid coupled to bovine serum albumin (BSA) as immunogen. Antiserum D12 recognized permethrin, cyfluthrin, cypermethrin, S-bioallethrin and the metabolite 1R trans-permethric acid with similar affinities. 50 % inhibition (50 % B/Bo) for permethrin occurred at 8| Hg/L; linearity ranged between 0.4 and 74 pg/L. The antisera D5 and D7 were obtained from preceding blood samples, and the antibodies showed different cross-reactivities, revealing the path of affinity maturation of the antiserum.

OriginalspracheEnglisch
Seiten (von - bis)781-795
Seitenumfang15
FachzeitschriftAnalytical Letters
Jahrgang28
Ausgabenummer5
DOIs
PublikationsstatusVeröffentlicht - 1995

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