Development of an Enzyme Immunoassay for the Determination of 2,4,6-Trinitrotoluene-Probing the Influences of Humic Acids

Claudia Keuchel; Ludwig Weil; Reinhard Niessner

doi:10.1117/12.140237

Development of an Enzyme Immunoassay for the Determination of 2,4,6-Trinitrotoluene-Probing the Influences of Humic Acids

Claudia Keuchel
, Ludwig Weil
, Reinhard Niessner

Natural Sciences

Technische Universität München

Publikation: Beitrag in Fachzeitschrift › Konferenzartikel › Begutachtung

4 Zitate (Scopus)

Abstract

A new enzyme immunoassay is described which can be used for the determination of the explosive 2,4,6-trinitrotoluene in water. The assay has a detection limit of 30 ng/l. The evaluation of cross-reactivities shows that the used antibody has a high selectivity for the trinitrophenyl moiety. Since humic substances, commonly occurring in natural waters, can interact with antibodies, the obtained results can be false positive. To reduce the negative influence of the humic substances the usually performed ELISA protocol has been modified by adding a solution of bovine serum albumin (0.5% in PBS) after the coating step.

Originalsprache	Englisch
Seiten (von - bis)	44-50
Seitenumfang	7
Fachzeitschrift	Proceedings of SPIE - The International Society for Optical Engineering
Jahrgang	1716
DOIs	https://doi.org/10.1117/12.140237
Publikationsstatus	Veröffentlicht - 1992
Veranstaltung	International Conference on Monitoring of Toxic Chemicals and Biomarkers 1992 - Berlin, Deutschland Dauer: 15 Juni 1992 → 19 Juni 1992

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title = "Development of an Enzyme Immunoassay for the Determination of 2,4,6-Trinitrotoluene-Probing the Influences of Humic Acids",

abstract = "A new enzyme immunoassay is described which can be used for the determination of the explosive 2,4,6-trinitrotoluene in water. The assay has a detection limit of 30 ng/l. The evaluation of cross-reactivities shows that the used antibody has a high selectivity for the trinitrophenyl moiety. Since humic substances, commonly occurring in natural waters, can interact with antibodies, the obtained results can be false positive. To reduce the negative influence of the humic substances the usually performed ELISA protocol has been modified by adding a solution of bovine serum albumin (0.5\% in PBS) after the coating step.",

author = "Claudia Keuchel and Ludwig Weil and Reinhard Niessner",

note = "Publisher Copyright: {\textcopyright} 1992 SPIE. All rights reserved.; International Conference on Monitoring of Toxic Chemicals and Biomarkers 1992 ; Conference date: 15-06-1992 Through 19-06-1992",

year = "1992",

doi = "10.1117/12.140237",

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T1 - Development of an Enzyme Immunoassay for the Determination of 2,4,6-Trinitrotoluene-Probing the Influences of Humic Acids

AU - Keuchel, Claudia

AU - Weil, Ludwig

AU - Niessner, Reinhard

PY - 1992

Y1 - 1992

N2 - A new enzyme immunoassay is described which can be used for the determination of the explosive 2,4,6-trinitrotoluene in water. The assay has a detection limit of 30 ng/l. The evaluation of cross-reactivities shows that the used antibody has a high selectivity for the trinitrophenyl moiety. Since humic substances, commonly occurring in natural waters, can interact with antibodies, the obtained results can be false positive. To reduce the negative influence of the humic substances the usually performed ELISA protocol has been modified by adding a solution of bovine serum albumin (0.5% in PBS) after the coating step.

AB - A new enzyme immunoassay is described which can be used for the determination of the explosive 2,4,6-trinitrotoluene in water. The assay has a detection limit of 30 ng/l. The evaluation of cross-reactivities shows that the used antibody has a high selectivity for the trinitrophenyl moiety. Since humic substances, commonly occurring in natural waters, can interact with antibodies, the obtained results can be false positive. To reduce the negative influence of the humic substances the usually performed ELISA protocol has been modified by adding a solution of bovine serum albumin (0.5% in PBS) after the coating step.

UR - https://www.scopus.com/pages/publications/0027311619

U2 - 10.1117/12.140237

DO - 10.1117/12.140237

M3 - Conference article

AN - SCOPUS:0027311619

SN - 0277-786X

VL - 1716

SP - 44

EP - 50

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - International Conference on Monitoring of Toxic Chemicals and Biomarkers 1992

Y2 - 15 June 1992 through 19 June 1992

ER -

Development of an Enzyme Immunoassay for the Determination of 2,4,6-Trinitrotoluene-Probing the Influences of Humic Acids

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