Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

Markus T. Berninger; Pouyan Mohajerani; Moritz Wildgruber; Nicolas Beziere; Melanie A. Kimm; Xiaopeng Ma; Bernhard Haller; Megan J. Fleming; Stephan Vogt; Martina Anton; Andreas B. Imhoff; Vasilis Ntziachristos; Reinhard Meier; Tobias D. Henning

doi:10.1016/j.pacs.2017.04.002

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

Markus T. Berninger, Pouyan Mohajerani, Moritz Wildgruber, Nicolas Beziere, Melanie A. Kimm, Xiaopeng Ma, Bernhard Haller, Megan J. Fleming, Stephan Vogt, Martina Anton, Andreas B. Imhoff, Vasilis Ntziachristos, Reinhard Meier, Tobias D. Henning

Professur für Sportorthopädie

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

19 Zitate (Scopus)

Abstract

The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 10⁶, 2 × 10⁵ and 2 × 10⁴ MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.

Originalsprache	Englisch
Seiten (von - bis)	37-47
Seitenumfang	11
Fachzeitschrift	Photoacoustics
Jahrgang	6
DOIs	https://doi.org/10.1016/j.pacs.2017.04.002
Publikationsstatus	Veröffentlicht - 1 Juni 2017

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10.1016/j.pacs.2017.04.002

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Berninger, M. T., Mohajerani, P., Wildgruber, M., Beziere, N., Kimm, M. A., Ma, X., Haller, B., Fleming, M. J., Vogt, S., Anton, M., Imhoff, A. B., Ntziachristos, V., Meier, R., & Henning, T. D. (2017). Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography. Photoacoustics, 6, 37-47. https://doi.org/10.1016/j.pacs.2017.04.002

@article{5514d0def0174fb0b1030dc129711f96,

title = "Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography",

abstract = "The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.",

keywords = "Cell labeling, Fluorescence molecular imaging, Intramyocardial injection, Mesenchymal stem cells, Multispectral optoacoustic tomography, Rabbit heart",

author = "Berninger, {Markus T.} and Pouyan Mohajerani and Moritz Wildgruber and Nicolas Beziere and Kimm, {Melanie A.} and Xiaopeng Ma and Bernhard Haller and Fleming, {Megan J.} and Stephan Vogt and Martina Anton and Imhoff, {Andreas B.} and Vasilis Ntziachristos and Reinhard Meier and Henning, {Tobias D.}",

note = "Publisher Copyright: {\textcopyright} 2017",

year = "2017",

month = jun,

day = "1",

doi = "10.1016/j.pacs.2017.04.002",

language = "English",

volume = "6",

pages = "37--47",

journal = "Photoacoustics",

issn = "2213-5979",

publisher = "Elsevier GmbH",

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TY - JOUR

T1 - Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

AU - Berninger, Markus T.

AU - Mohajerani, Pouyan

AU - Wildgruber, Moritz

AU - Beziere, Nicolas

AU - Kimm, Melanie A.

AU - Ma, Xiaopeng

AU - Haller, Bernhard

AU - Fleming, Megan J.

AU - Vogt, Stephan

AU - Anton, Martina

AU - Imhoff, Andreas B.

AU - Ntziachristos, Vasilis

AU - Meier, Reinhard

AU - Henning, Tobias D.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.

AB - The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.

KW - Cell labeling

KW - Fluorescence molecular imaging

KW - Intramyocardial injection

KW - Mesenchymal stem cells

KW - Multispectral optoacoustic tomography

KW - Rabbit heart

UR - http://www.scopus.com/inward/record.url?scp=85019168660&partnerID=8YFLogxK

U2 - 10.1016/j.pacs.2017.04.002

DO - 10.1016/j.pacs.2017.04.002

M3 - Article

AN - SCOPUS:85019168660

SN - 2213-5979

VL - 6

SP - 37

EP - 47

JO - Photoacoustics

JF - Photoacoustics

ER -

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography

Abstract

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