Detection and quantification of mRNA expression of α- and β-adrenergic receptor subtypes in the mammary gland of dairy cows

T. Inderwies, M. W. Pfaffl, H. H.D. Meyer, J. W. Blum, R. M. Bruckmaier

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

40 Zitate (Scopus)

Abstract

Adrenergic receptors are pharmacologically classified into the receptor types α1, α2, β1, β2, and β3. Structural differences and varying affinities in radioligand binding studies lead to a further classification of α1- and α2-receptors into subtypes which are termed α1A (formerly α1C), α1B, and α1D (formerly α1AD), and α2AD, α2B, and α2C, respectively, mRNA expression of all but one α-adrenergic receptor subtypes and of all β-adrenergic receptor types was measured quantitatively in total RNA extracted from mammary tissue of 10 lactating dairy cows by real-time reverse transcription (RT) polymerase chain reaction (PCR). mRNA expression of α1-adrenergic receptors was highest for the α1A-subtype followed by α1B, whereas the α1D-Subtype could not be detected. The highest mRNA expression of α2-adrenergic receptors was found for the α2AD-subtype, followed by α2B and α2C. Within the β-adrenergic receptors, the β2-receptor type was most highly expressed, followed by β1 and β3. In conclusion, eight of nine adrenergic receptors classified to date were detected and relatively quantified in the mammary gland of dairy cows.

OriginalspracheEnglisch
Seiten (von - bis)123-135
Seitenumfang13
FachzeitschriftDomestic Animal Endocrinology
Jahrgang24
Ausgabenummer2
DOIs
PublikationsstatusVeröffentlicht - März 2003

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