Demonstration of flavonoid enzymes in cyanic and acyanic pelargonium-zonale-hybrids

L. Jánvári, R. Hassenpflug, G. Forkmann, K. Olbricht

Publikation: Beitrag in Buch/Bericht/KonferenzbandKonferenzbeitragBegutachtung

2 Zitate (Scopus)

Abstract

The cyanic and acyanic colour of Pelargonium cultivars is the result of the presence or absence of anthocyanins in the flowers. Instead of anthocyanins, acyanic flowers contain different types and amounts of flavonoids leading to white or cream colour. Our aim was to demonstrate the enzymes of the anthocyanin pathway and to identify possible blocks in the main enzymatic steps. We investigated four enzymes, chalcone synthase (CHS), flavanone 3β-hydroxylase (FHT), dihydroflavonol 4-reductase (DFR) and flavonol synthase (FLS). The first three enzymes lead to the production of leucoanthocyanidins, the substrates of the anthocyanidin synthase (ANS), and FLS is responsible for flavonol synthesis. In spite of many examples of testing the above enzymes in numerous species, up to now, Pelargonium proved to be a recalcitrant plant for enzymatic studies. Nevertheless, we detected significant differences in enzyme activity between cultivars and between flower developmental stages of the same cultivar. The acyanic cultivars studied seem to be blocked late in the anthocyanin pathway. Our results may serve as a basis for further genetic and biochemical characterisation of Pelargonium plants.

OriginalspracheEnglisch
TitelXX International Eucarpia Symposium, Section Ornamentals, Strategies for New Ornamentals - Part II
Herausgeber (Verlag)International Society for Horticultural Science
Seiten83-90
Seitenumfang8
ISBN (Print)9789066058255
DOIs
PublikationsstatusVeröffentlicht - 27 Feb. 2002

Publikationsreihe

NameActa Horticulturae
Band572
ISSN (Print)0567-7572

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