TY - JOUR
T1 - Comparison of the proteomes of three yeast wild type strains
T2 - CEN.PK2, FY1679 and W303
AU - Rogowska-Wrzesinska, Adelina
AU - Larsen, Peter Mose
AU - Blomberg, Anders
AU - Görg, Angelika
AU - Roepstorff, Peter
AU - Norbeck, Joakim
AU - Fey, Stephen John
N1 - Funding Information:
Rogowska-Wrzesinska Adelina [email protected] 1 Larsen Peter Mose 1 Blomberg Anders 2 Görg Angelika 3 Roepstorff Peter 4 Norbeck Joakim 5 Fey Stephen John 1 1 Centre for Proteome Analysis in Life Sciences University of Southern Denmark International Science Park Odense Forskerparken 10B Odense M 5230 Denmark sdu.dk 2 Deptartment of Cell and Molecular Biology – Microbiology Lundbergs Laboratory Göteborg University Medicinaregatan 9C Göteborg 405 30 Sweden gu.se 3 Technical University of Munich Institute of Food Technology and Analytical Chemistry Proteomics Group Freising-Weihenstephan D-85350 Germany mytum.de 4 Department of Biochemistry and Molecular Biology University of Southern Denmark Odense University, Campusvej 55 Odense M 5230 Denmark sdu.dk 5 Institut fuer Biochemie und Molekulare Zellbiologie Universität Wien Vienna Dr-Bohrgasse 9 A-1030 Austria univie.ac.at 2001 2 4 207 225 01 05 2001 26 06 2001 2001 Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Yeast deletion strains created during gene function analysis projects very often show drastic phenotypic differences depending on the genetic background used. These results indicate the existence of important molecular differences between the CEN.PK2, FY1679 and W303 wild type strains. To characterise these differences we have compared the protein expression levels between CEN.PK2, FY1679 and W303 strains using twodimensional gel electrophoresis and identified selected proteins by mass spectrometric analysis. We have found that FY1679 and W303 strains are more similar to each other than to the CEN.PK2 strain. This study identifies 62 proteins that are differentially expressed between the strains and provides a valuable source of data for the interpretation of yeast mutant phenotypes observed in CEN.PK2, FY1679 and W303 strains. http://dx.doi.org/10.13039/501100000780 European Commission BIO4CT972361 Danish National Research Foundation
PY - 2001
Y1 - 2001
N2 - Yeast deletion strains created during gene function analysis projects very often show drastic phenotypic differences depending on the genetic background used. These results indicate the existence of important molecular differences between the CEN.PK2, FY1679 and W303 wild type strains. To characterise these differences we have compared the protein expression levels between CEN.PK2, FY1679 and W303 strains using two-dimensional gel electrophoresis and identified selected proteins by mass spectrometric analysis. We have found that FY1679 and W303 strains are more similar to each other than to the CEN.PK2 strain. This study identifies 62 proteins that are differentially expressed between the strains and provides a valuable source of data for the interpretation of yeast mutant phenotypes observed in CEN.PK2, FY1679 and W303 strains.
AB - Yeast deletion strains created during gene function analysis projects very often show drastic phenotypic differences depending on the genetic background used. These results indicate the existence of important molecular differences between the CEN.PK2, FY1679 and W303 wild type strains. To characterise these differences we have compared the protein expression levels between CEN.PK2, FY1679 and W303 strains using two-dimensional gel electrophoresis and identified selected proteins by mass spectrometric analysis. We have found that FY1679 and W303 strains are more similar to each other than to the CEN.PK2 strain. This study identifies 62 proteins that are differentially expressed between the strains and provides a valuable source of data for the interpretation of yeast mutant phenotypes observed in CEN.PK2, FY1679 and W303 strains.
KW - CEN.PK2
KW - FY1679
KW - Protein expression
KW - Proteomics
KW - Saccharomyces cerevisiae
KW - Two-dimensional gel electrophoresis
KW - W303
UR - http://www.scopus.com/inward/record.url?scp=0034875386&partnerID=8YFLogxK
U2 - 10.1002/cfg.94
DO - 10.1002/cfg.94
M3 - Article
AN - SCOPUS:0034875386
SN - 1531-6912
VL - 2
SP - 207
EP - 225
JO - Comparative and Functional Genomics
JF - Comparative and Functional Genomics
IS - 4
ER -