TY - GEN
T1 - Comparison of immunochemical and HPLC-determination of polycyclic aromatic hydrocarbons in groundwater samples of a former manufactured gas plant site
AU - Knopp, Dietmar
AU - Vaananen, Virpi
AU - Niessner, Reinhard
PY - 1995
Y1 - 1995
N2 - Groundwater contamination and PAH-removal with a special water purification facility were monitored over several months by a PAH-immunoassay as well as by HPLC. In the enzyme- linked immunosorbent assay (ELISA) a polyclonal rabbit antiserum was applied. As test format an indirect competitive microtiter plate assay was used. The water samples could be directly measured without any extraction and purification steps. With the ELISA, the limit value for the sum of PAHs of 0.2 ppb as set by the German Drinking Water Act was certainly reached. Including 114 samples no false negative (defined as a measured concentration below 0.2 ppb) but 20 (17.5%) false positive (defined as a measured concentration above 0.2 ppb) samples were found with the immunoassay. Supposing the immunochemical results to be approximate values of the total PAH concentration then these data correlate very well (r equals 0.82, n equals 114) with the sum of the 16 EPA PAHs as measured by HPLC in this study. Therefore, the ELISA could be used as a rapid screening technique to diminish the sample number for HPLC.
AB - Groundwater contamination and PAH-removal with a special water purification facility were monitored over several months by a PAH-immunoassay as well as by HPLC. In the enzyme- linked immunosorbent assay (ELISA) a polyclonal rabbit antiserum was applied. As test format an indirect competitive microtiter plate assay was used. The water samples could be directly measured without any extraction and purification steps. With the ELISA, the limit value for the sum of PAHs of 0.2 ppb as set by the German Drinking Water Act was certainly reached. Including 114 samples no false negative (defined as a measured concentration below 0.2 ppb) but 20 (17.5%) false positive (defined as a measured concentration above 0.2 ppb) samples were found with the immunoassay. Supposing the immunochemical results to be approximate values of the total PAH concentration then these data correlate very well (r equals 0.82, n equals 114) with the sum of the 16 EPA PAHs as measured by HPLC in this study. Therefore, the ELISA could be used as a rapid screening technique to diminish the sample number for HPLC.
UR - http://www.scopus.com/inward/record.url?scp=0029517067&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0029517067
SN - 0819418625
SN - 9780819418623
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 531
EP - 538
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Vo-Dinh, Tuan
T2 - Environmental Monitoring and Hazardous Waste Site Remediation
Y2 - 19 June 1995 through 21 June 1995
ER -