Circulating microRNAs as prognostic therapy biomarkers in head and neck cancer patients

I. Summerer, K. Unger, H. Braselmann, L. Schuettrumpf, C. Maihoefer, P. Baumeister, T. Kirchner, M. Niyazi, E. Sage, H. M. Specht, G. Multhoff, S. Moertl, C. Belka, H. Zitzelsberger

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

117 Zitate (Scopus)

Abstract

The prediction of therapy response in head and neck squamous cell cancer (HNSCC) requires biomarkers, which are also a prerequisite for personalised therapy concepts. The current study aimed to identify therapy-responsive microRNAs (miRNAs) in the circulation that can serve as minimally invasive prognostic markers for HNSCC patients undergoing radiotherapy.Methods:We screened plasma miRNAs in a discovery cohort of HNSCC patients before therapy and after treatment. We further compared the plasma miRNAs of the patients to age-and sex-matched healthy controls. All miRNAs identified as biomarker candidates were then confirmed in an independent validation cohort of HNSCC patients and tested for correlation with the clinical outcome.Results:We identified a signature of eight plasma miRNAs that differentiated significantly (P=0.003) between HNSCC patients and healthy donors. MiR-186-5p demonstrated the highest sensitivity and specificity to classify HNSCC patients and healthy individuals. All therapy-responsive and patient-specific miRNAs in plasma were also detectable in tumour tissues derived from the same patients. High expression of miR-142-3p, miR-186-5p, miR-195-5p, miR-374b-5p and miR-574-3p in the plasma correlated with worse prognosis.Conclusions:Circulating miR-142-3p, miR-186-5p, miR-195-5p, miR-374b-5p and miR-574-3p represent the most promising markers for prognosis and therapy monitoring in the plasma of HNSCC patients. We found strong evidence that the circulating therapy-responsive miRNAs are tumour related and were able to validate them in an independent cohort of HNSCC patients.

OriginalspracheEnglisch
Seiten (von - bis)76-82
Seitenumfang7
FachzeitschriftBritish Journal of Cancer
Jahrgang113
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - 30 Juni 2015

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