Characterization of cells and bacteria by photophoretic velocimetry

Clemens Helmbrecht, Reinhard Niessner, Christoph Haisch

Publikation: Beitrag in Buch/Bericht/KonferenzbandKonferenzbeitragBegutachtung

2 Zitate (Scopus)

Abstract

The migration induced by intensive light is termed photophoresis. We could show that the evaluation of light-induced velocities of microparticles, bacteria and cells suspended in water is valuable for the prediction of their intrinsic properties. Two different laser setups were evaluated for photophoretic migration, a He-Ne laser (P = 45 mW, λ = 633 nm) and a diode-pumped cw-Nd:YAG (P = 1.1 W, λ = 532 nm). When analyzing the migration behavior of particles, we find significant differences depending on both, geometrical size and refractive index. We describe migration of PS particles of different size as well as with different refractive index but same diameter, SiO 2 and melamine resin. The potential for the separation of biological matter is shown as velocity distributions of heat killed bacteria of Escherichia coli, Salmonella enteritidis, and baker's yeast is reported.

OriginalspracheEnglisch
TitelImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
DOIs
PublikationsstatusVeröffentlicht - 2008
VeranstaltungImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI - San Jose, CA, USA/Vereinigte Staaten
Dauer: 21 Jan. 200823 Jan. 2008

Publikationsreihe

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Band6859
ISSN (Print)1605-7422

Konferenz

KonferenzImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
Land/GebietUSA/Vereinigte Staaten
OrtSan Jose, CA
Zeitraum21/01/0823/01/08

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