Blue LAMP supports the selection of prunus domestica genotypes with hypersensitivity resistance to the plum pox virus

The screening of genotypes derived from the Weihenstephan plum breeding program for hypersensitivity resistance against the Plum pox virus is based on the monitoring of the hypersensitive reaction. Biological screening is supported by the Blue LAMP protocol for the detection of PPV because of its straightforward procedure and reliability. The specificity of the LAMP technology also allows for PPV strain differentiation which is important to validate the stability of hypersensitivity against all known subgroups of PPV during the selection process of resistant genotypes. The seven primer sets designed for this purpose were specific to each one of the seven PPV strains. Unspecific amplification observed in initial experiments was inhibited by adjusting the trehalose concentration.