Biosynthesis of orchid-like volatile methyl jasmonate in tea (Camellia sinensis) leaves in response to multiple stresses during the shaking process of oolong tea

Yingying Feng, Jingming Wang, Yifan Zhao, Mengting Zhang, Zixiang Zhou, Yating Li, Yunqing Hu, Yi Wu, Zhihui Feng, Wilfried Schwab, Xiaochun Wan, Chuankui Song

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

19 Zitate (Scopus)

Abstract

Methyl jasmonate (MeJA), a significant fatty acid derivative, contributes to the main floral aroma of oolong tea. However, in tea plants, the mechanism of MeJA biosynthesis and the enzymes that participate in its formation is still unclear. In this study, the full length of the first jasmonic acid carboxyl methyltransferase (JMT) gene from Camellia sinensis was amplified and the biochemical function of the recombinant C. sinensis JMT (CsJMT) protein was studied after coexpression in Escherichia coli. The recombinant CsJMT protein could transfer the methyl groups to jasmonic acid to produce orchid-like volatile 1R, 2R-MeJA in vitro. Volatile 1R, 2R-MeJA was detected by gas chromatography–mass spectrometry during the shaking process of oolong tea, and the accumulation of 1R, 2R-MeJA was found to correlate with CsJMT expression. Further investigation showed that the mechanical injury and low-temperature stress could significantly induce CsJMT expression and MeJA accumulation after harvest. These results demonstrated that CsJMT catalyzed the production of MeJA in tea plants and provided a basis for studying the formation and accumulation of orchid-like volatile MeJA during the shaking process of oolong tea.

OriginalspracheEnglisch
Aufsatznummer111184
FachzeitschriftLWT
Jahrgang143
DOIs
PublikationsstatusVeröffentlicht - Mai 2021

Fingerprint

Untersuchen Sie die Forschungsthemen von „Biosynthesis of orchid-like volatile methyl jasmonate in tea (Camellia sinensis) leaves in response to multiple stresses during the shaking process of oolong tea“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren